FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12735
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCG5704.47585394581861No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4313.384373773066353No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCT3252.5520219866509617No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2752.159403219473891No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.8637612877895563No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGG970.7616804083235179No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG960.7538280329799765No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC930.7302709069493523No Hit
ATTGCGCAATGAACCCCAGGGGGACGTCAGCCAAACTCCGAATGCCGGTA890.6988614055751865No Hit
ATTGCGCAATGTCTGGATCGGGTGCGTAAAGGAGAAGGGCACGGCGCTGC850.6674519042010209No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT680.5339615233608166No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCAT630.49469964664310956No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACC580.45543776992540247No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT560.4397330192383196No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCG550.43188064389477815No Hit
ATTGCGCAATGAAGGACTCGGGGATGTCGGCTCGTCTCATCCTGGAGCTG540.4240282685512367No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT520.40832351786415394No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT460.3612092658029054No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG410.32194738908519827No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG400.3140950137416569No Hit
ATTGCGCAATGAACTCCAGGGGGACGTCAGCCAAACTCCGAATGCCGGTA390.30624263839811544No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG360.2826855123674912No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC340.2669807616804083No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC320.25127601099332547No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG310.24342363564978406No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA290.22771888496270123No Hit
ATTGCGCAATGAGTGGAATGGGGCTCTTTTACCGAGACCCGCCAACATGG280.2198665096191598No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCA280.2198665096191598No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC270.21201413427561835No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT270.21201413427561835No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCT270.21201413427561835No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC260.20416175893207697No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT260.20416175893207697No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG250.19630938358853553No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCG250.19630938358853553No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC230.1806046329014527No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC230.1806046329014527No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAC220.17275225755791127No Hit
ATTGCGCAATGACAAGACCGGGGGTTGTCCGGGGCTAAGTGTGTGGACTG200.15704750687082844No Hit
ATTGCGCAATGACCAGCCCGGGGGTTGTCCGGGGCTAAGTGTGTGGACTG200.15704750687082844No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC190.149195131527287No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT190.149195131527287No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT170.13349038084020415No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGTC160.12563800549666274No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT160.12563800549666274No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACC160.12563800549666274No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCAGCG160.12563800549666274No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT160.12563800549666274No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT140.1099332548095799No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG140.1099332548095799No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT140.1099332548095799No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCCT140.1099332548095799No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTCT130.10208087946603848No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCAGTC130.10208087946603848No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT130.10208087946603848No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC130.10208087946603848No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG130.10208087946603848No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT130.10208087946603848No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)