FastQCFastQC Report
Tue 7 May 2024
EGAF00007823586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12735
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTA9847.726737338044759No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5394.2324303101688265No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3312.5991362387122106No Hit
CTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGG1150.9030231645072634No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTA970.7616804083235179No Hit
CTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTC730.5732234000785238No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.5496662740478995No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTG670.5261091480172753No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCG580.45543776992540247No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC510.4004711425206125No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.3612092658029054No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC450.35335689045936397No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG420.3297997644287397No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA420.3297997644287397No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGTCGCC400.3140950137416569Illumina Single End PCR Primer 1 (100% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG390.30624263839811544No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA320.25127601099332547No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA310.24342363564978406No Hit
ATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGG310.24342363564978406No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG300.23557126030624262No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC290.22771888496270123No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATT260.20416175893207697No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG250.19630938358853553No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGC240.18845700824499412No Hit
CTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGTTCTCGG240.18845700824499412No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT230.1806046329014527No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG220.17275225755791127No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA210.16489988221436985No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.16489988221436985No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGTGGCC200.15704750687082844Illumina Single End PCR Primer 1 (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC200.15704750687082844No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCC200.15704750687082844No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT200.15704750687082844No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC190.149195131527287No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC190.149195131527287No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATC170.13349038084020415No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTT170.13349038084020415No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT160.12563800549666274No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGGCGCC150.11778563015312131Illumina Single End PCR Primer 1 (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA150.11778563015312131No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCG150.11778563015312131No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGA150.11778563015312131No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA140.1099332548095799No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA140.1099332548095799No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGGA140.1099332548095799No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT140.1099332548095799No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA140.1099332548095799No Hit
TCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAG140.1099332548095799No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAAT140.1099332548095799No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA130.10208087946603848No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAA130.10208087946603848No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGGGGCC130.10208087946603848No Hit
CTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGTTCTC130.10208087946603848No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAATGT130.10208087946603848No Hit
ATCTATCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTA130.10208087946603848No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC130.10208087946603848No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)