FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007823587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7930
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCG3654.602774274905422No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2803.5308953341740232No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCT2383.001261034047919No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2112.66078184110971No Hit
ATTGCGCAATGGGTTTCACGGGCCGGACATTAACTGGACTGATCAACGTG1231.5510718789407314No Hit
ATTGCGCAATGTCCTTCCCGGGGTGATGCGGATCGAAAACAAGTAGAGAA881.1097099621689785No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.7944514501891551No Hit
ATTGCGCAATGGATGGGGAGGGTAAAGATTGTGAAAAGGAGAAAGAGGAA610.7692307692307693No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCG540.6809583858764187No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCAT430.5422446406052964No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC310.3909205548549811No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT270.34047919293820933No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT270.34047919293820933No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG240.3026481715006305No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG220.27742749054224464No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT210.2648171500630517No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC200.25220680958385877No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAT200.25220680958385877No Hit
ATTGCGCAATGCTTCCAGTGGGGGGTATGGCTGTTCGCCATTTAAAGTGG180.22698612862547288No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG170.21437578814627994No Hit
ATTGCGCAATGGATCTCGTGGGGATGGAGTCTGGTGCCAAGGGTTGTGAA170.21437578814627994No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC170.21437578814627994No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC160.201765447667087No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA160.201765447667087No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG150.18915510718789408No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCG150.18915510718789408No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCAGCG150.18915510718789408No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT140.17654476670870115No Hit
ATTGCGCAATGGATCGTCCGGGGGCTCTCCGCCCCGGTATCAAGGCCACC140.17654476670870115No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA130.16393442622950818No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAT120.15132408575031525No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATG120.15132408575031525No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT110.13871374527112232No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT110.13871374527112232No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC110.13871374527112232No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCT110.13871374527112232No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT110.13871374527112232No Hit
ATTGCGCAATCTGTCTCTTATCTGTCTCTTATACACATCTCCGAGCCCAC100.12610340479192939No Hit
ATTGCGCAATGTGGGGGAGGGGGCCAAGAAAATTATTGCTTGCCCTGAAA100.12610340479192939No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT100.12610340479192939No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTC90.11349306431273644No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT90.11349306431273644No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATGG90.11349306431273644No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCTCG90.11349306431273644No Hit
ATTGCGCAATGCGTCTCCTGGGGAAAAAAGATAAAAAAAAAAACAAAAAC90.11349306431273644No Hit
ATTGCGCAATGGGATCATCGGGATGCAAAATATGGTCAAAGAATTCAAGA80.1008827238335435No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG80.1008827238335435No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCTC80.1008827238335435No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCGCG80.1008827238335435No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC80.1008827238335435No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA80.1008827238335435No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)