FastQCFastQC Report
Thu 5 Jan 2023
EGAF00007826418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007826418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21297994
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC3497381.6421170932811795TruSeq Adapter, Index 7 (100% over 50bp)
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC3154251.4810080235725487No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT2880761.352596868982121No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG1729610.812099956455993Clontech Universal Primer Mix Short (95% over 21bp)
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG1257130.5902574674403608No Hit
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC1140860.5356654715932402No Hit
AATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTC490960.23051936252775734No Hit
CTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCAA488990.2295943927864756No Hit
GGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGA427100.2005353180210305No Hit
ATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGC403450.18943098584777515Clontech Universal Primer Mix Short (95% over 21bp)
TAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCT387970.18216269569800797No Hit
AGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGC293780.13793787339784208No Hit
AATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGG292630.1373979164422715Clontech Universal Primer Mix Short (95% over 21bp)
ACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCG278310.13067427852594946No Hit
TACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTC273390.12836420181168234No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG267110.12541556730647968No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG232660.10924033502873558TruSeq Adapter, Index 7 (100% over 49bp)
AGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCC218300.1024979160009154No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT214210.10057754735023403No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)