FastQCFastQC Report
Thu 5 Jan 2023
EGAF00007826419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007826419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21297994
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC4588042.154212269944296No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3747301.759461477921348Illumina Single End PCR Primer 1 (100% over 50bp)
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT1690150.7935723899631111No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG1558230.7316322842423564No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG1127300.5292986747953822Clontech Universal Primer Mix Short (95% over 21bp)
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC725240.3405203325721662No Hit
GGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGA548510.25754068669565783No Hit
AGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGC361610.1697859432207559No Hit
CTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCAA327120.1535919298315137No Hit
AGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCC311290.14615930495613813No Hit
AATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTC277840.1304536004658467No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC250410.11757445325602026Illumina Single End PCR Primer 1 (100% over 50bp)
GCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGA239080.11225470342418165No Hit
GAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCG234730.11021225754876257No Hit
ATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGC223640.10500519438591258Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)