FastQCFastQC Report
Thu 5 Jan 2023
EGAF00007826472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007826472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77425294
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGCTATCTCGTTTGC1340480.17313205165226755TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGCTATCTCGTATGC1090370.14082865478043907TruSeq Adapter, Index 14 (97% over 38bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA874180.11290625515739082No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT812780.10497603018465776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)