FastQCFastQC Report
Sat 4 Feb 2023
EGAF00007826517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007826517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185943121
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCCCGA6393482034.384073826533225No Hit
CCAAGATG4823594825.94123823489012No Hit
GTTTATCT3732470820.073185713603248No Hit
TACGTGAC3441533618.508528745196227RNA PCR Primer, Index 17 (100% over 8bp)
GGTTATCT2405500.1293675177152695No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)