FastQCFastQC Report
Sat 4 Feb 2023
EGAF00007826556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007826556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153888504
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGAGT5126125833.31064807803967No Hit
TACGTTCA3658108823.771163569177332No Hit
CTATAGTG3437244422.335940051766308Clontech Universal Primer Mix Short (100% over 8bp)
AGTCCCAC3016135019.599482232928846No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)