FastQCFastQC Report
Sat 4 Feb 2023
EGAF00007826580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007826580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163524279
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTTGAT4799320229.349282133205428No Hit
AGCGCCTA4354383926.628363241399768No Hit
GCTCAACC4278018326.161364698632916No Hit
CAAAGTGG2719757916.632135097198624ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
GTGTTGAT3477570.21266383324032267No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)