FastQCFastQC Report
Fri 10 May 2024
EGAF00007826586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007826586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166381786
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACGCC6123462536.80368294640136No Hit
CTAGGTGA5238302031.48362645896829RNA PCR Primer, Index 38 (100% over 8bp)
AGCCAATT4997731030.037729009592436No Hit
TCGTTCAG10418420.6261755117834833No Hit
AGCCAATA1709720.10275884404798973No Hit
NATACGCC1708720.10269874131535048No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)