FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007829406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007829406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10931188
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN409720.3748174489360168No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG234150.2142036162949535Illumina Single End PCR Primer 1 (100% over 50bp)
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC126830.11602581530936985No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG110020.10064779784228392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)