FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007829415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007829415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22675612
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6104712.6921919461313766TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG335330.1478813449445157TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAT287760.12690285933627724TruSeq Adapter, Index 9 (97% over 36bp)
CCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTTCTTCTAAAAGTATGTTTT252410.11131342342601382No Hit
GCCCTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTT230140.10149229930376301No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)