FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007829451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007829451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22638796
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9384674.14539271434753TruSeq Adapter, Index 7 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA557540.24627634791178823TruSeq Adapter, Index 7 (97% over 38bp)
CCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTTCTTCTAAAAGTATGTTTT480750.21235669953472788No Hit
CTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTTCTT420490.18573867620875245No Hit
GCCCTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTT386690.17080855359975858No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTTCAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAA288040.12723291468327202TruSeq Adapter, Index 7 (97% over 38bp)
CTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCCAAA241620.10672829067411536No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)