FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007829453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007829453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18675534
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5320242.848775301418423TruSeq Adapter, Index 9 (97% over 37bp)
CCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTTCTTCTAAAAGTATGTTTT413730.22153583399542953No Hit
GCCCTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTT382150.20462600962307154No Hit
CTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTTCTT332460.17801900604287943No Hit
CTCATTTTTTCAGTGCTATTTTATCCAATTTTTGGCTTTATATTTTTCTATCTTCTATACTTCTCCAATACTTGT230640.1234984766700647No Hit
CTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCCAAA225850.12093362363828526No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA193600.10366504111743204TruSeq Adapter, Index 9 (97% over 37bp)
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAG187390.10033983499481193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)