FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007829457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007829457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58169058
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCAAGTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA26914784.626992584270489TruSeq Adapter, Index 6 (97% over 38bp)
CCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTTCTTCTAAAAGTATGTTTT1341080.23054868792958622No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCAAGTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1130830.19440404209399437TruSeq Adapter, Index 6 (97% over 38bp)
CTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTTCTT1033280.1776339579024986No Hit
GCCCTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTT982840.16896268115601942No Hit
CTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCCAAA773710.13301057754794654No Hit
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAG667900.11482049442849838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)