FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007829463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007829463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18616907
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3177801.706943049132705TruSeq Adapter, Index 6 (97% over 36bp)
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAG337980.18154465723011884No Hit
CCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTTCTTCTAAAAGTATGTTTT238640.1281845582620142No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG231800.12451047856660614No Hit
GCCCTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTT214320.11512116378945224No Hit
CTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTTCTT211760.11374606963444572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)