FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007829467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007829467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21723722
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4353892.0042099599691063TruSeq Adapter, Index 1 (97% over 39bp)
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAG545450.2510849660108889No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG349010.16065847279761727No Hit
CCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTTCTTCTAAAAGTATGTTTT258260.11888386345581112No Hit
CTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTTCTT234620.10800175034462325No Hit
GCCCTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTT220730.1016078184023898No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)