FastQCFastQC Report
Sun 21 May 2023
EGAF00007832349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125939
Sequences flagged as poor quality0
Sequence length247-250
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA8895870.63578399066215No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC1486211.80095125417861No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC36962.934754126997991No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC19131.518989351987867No Hit
GGAAGTAAAAGTCGTAACAAGGCGAAAGACGGCACGACGGATGAGATGAT18381.4594367114237845No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC13921.1052970088693734No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCG9060.719395898014118No Hit
GGAAGTAAAAGTCGTAACAAGCCACAGAAGAAATGAACGATCGTAGGGAT6270.4978600751157306No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGAAACAGAATCCGAAAAGAGCACA5820.4621284907772811No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG5750.45657024432463333No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG5020.3986056741755929No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCTGGCA4900.3890772516853397No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCGTCGTTACC4900.3890772516853397No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC4540.36049198421458006No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC4460.3541397025544113No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC3830.30411548448058184No Hit
GGAAGTAAAAGTCGTAACAAGGACAACTGGAAGCAGTACATCGACGGACT2570.20406704833292308No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC2400.19056844980506438No Hit
GGAAGTAAAAGTCGTAACAAGGCGCAGCAGGCAGCACAAGAGAAAGAAAA1870.14848458380644597No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC1730.13736809090115057No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACAACTCTCCGGCA1630.12942773882593955No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC1370.1087828234303909No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGAA1350.1071947530153487No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC1260.10004843614765879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)