FastQCFastQC Report
Sun 21 May 2023
EGAF00007832352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206495
Sequences flagged as poor quality0
Sequence length235-238
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT5832828.246688781810697No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT5247025.40981621831037No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT195019.443812198842586No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG186569.034601322065908No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT86104.1695924840795175No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG71493.4620692994987774No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG66153.2034673963049953No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT33471.6208624906172062No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT21881.0595898205767693No Hit
GCTGCGTTCTTCATCGATGCGTGACTGTTGGGCCCCACTGCTGCGACTCT21361.0344076127751278No Hit
GCTGCGTTCTTCATCGATGCCACCACCTCAATGCCATCTCGCTGATCTGG17420.8436039613549965No Hit
GCTGCGTTCTTCATCGATGCCTCCCCAGTATCTCCTGCTCTCTTTTGCAT15400.7457807695101576No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT14800.7167243758928787No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT10670.5167195331606091No Hit
GCTGCGTTCTTCATCGTTTCGAAACGCCTAGGCATCCGCCGTATGCCCTT9840.47652485532337346No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA7420.3593307344003487No Hit
GCTGCGTTCTTCATCGACTCGAAACGCCTAGGCATCCACCGTATGCCCTT6700.32446306205961406No Hit
GCTGCGTTCTTCATCGATGTCGGAACCAAGAGATCCGTTGTTGAAAGTTT5120.24794789220077967No Hit
GCTGCGTTCTTCATCGATGCACACATGGGGGCCACGTCAGGTGACCACTC5070.24552652606600645No Hit
GCTGCGTTCTTCATCGATGCAGGTGAAGAGGTCTATGGCGGACAGGTCGT4770.230998329257367No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG4460.2159858592217729No Hit
TCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT4410.21356449308699968No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT4170.2019419356400881No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT3400.16465289716458026No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTCCACTTCGCAAATCAAAATAA3140.1520617932637594No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTTCCGTCTTTTCTTCTTGTTT2880.13947068936293858No Hit
TCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT2810.13608077677425603No Hit
GCTGCGTTCTTCATCGACTCCTGACGCCAAGGCATCCACCGTATGCCTTT2630.12736385868907238No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC2480.12009976028475265No Hit
GCTGCGTTCTTCATCGATGCGGTGACCCCCCTGGTCTCTCAGTCCCAGCC2360.1142884815612969No Hit
GCTGCGTTCTTCATCGATGCCGCAATGGTAGGGCATTTAGGCTCAGCAGC2330.11283566188043295No Hit
GCTGCGTTCTTCATCGATGCCAGCGCTGCGGGGTCGGGGATATCGGCGAG2320.1123513886534783No Hit
GCTGCGTTCTTCATCGATGTCTTCCTTTGTAGCATCAGCCGGGAAAGTCA2090.1012131044335214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)