FastQCFastQC Report
Sun 21 May 2023
EGAF00007832355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271289
Sequences flagged as poor quality0
Sequence length247-250
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC19751272.80501605299146No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC138565.107468419287181No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC116994.3123753635422No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC65762.4239832798233616No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG27321.0070441484910926No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC24580.9060448451651191No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC18380.6775062755953982No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC16850.6211088543951284No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC15160.5588136636575756No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCCAGTGCTTG12840.4732960053669703No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA12080.44528160006487544No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC12070.44491298946879526No Hit
GGAAGTAAAAGTCGTAACAAGGACAGAGCAACGTATCAGGCGGCGGTGGC12050.4441757682766349No Hit
GGAAGTAAAAGTCGTAACAAGGTGCCCAAATGAAGCACGCCGGGGCGGAC10690.3940447272097284No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC10240.3774572503861196No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG10140.37377114442531767No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC9680.3568150570056287No Hit
GGAAGTAAAAGTCGTAACAAGCACACAATGGTAAGAAAGAGCTACCTTGA9630.3549720040252277No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC9430.3475997921036238No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCATAGGTGAACCTGCGGAAGGATC7860.28972792851903323No Hit
GGAAGTAAAAGTCGTAACAAGGGGAAGTAGACCTGGCCCTCTTCCAGGGC5810.21416275632259327No Hit
GGAAGTAAAAGTCGTAACAAGGGTTAGTAGATGTTTTAAAATTTTCATTT5750.21195109274611207No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC5540.20421027022842797No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA4760.17545864373417278No Hit
GGAAGTAAAAGTCGTAACAAGGACGAGGTGGACATCCGCGTGGCGATCAG4190.15444783975760168No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC3870.14265230068303542No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTACTGATTTATTGGTAGCATCCTCC3040.11205762120837927No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA2800.10321096690245458No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)