FastQCFastQC Report
Sun 21 May 2023
EGAF00007832373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75456
Sequences flagged as poor quality0
Sequence length247-250
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC3601247.725826972010175No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC1114814.774173027989823No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA33074.382686598812553No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA32554.3137722646310435No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC26953.571617896522477No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGTTGAAAAAGCTGGTAATTGTCAT21752.8824745547073793No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC19052.5246501272264634No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGACAAGCTGATGATTGTGGGAGCAT16242.1522476675148434No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC13111.7374363867684477No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC13051.7294847328244274No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC9131.2099766751484309No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC8651.146363443596268No Hit
GGAAGTAAAAGTCGTAACAAGGCGAAAGACGGCACGACGGATGAGATGAT4110.5446882951653944No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGGTCAGCTAATCGACGCAATC3380.4479431721798134No Hit
GGAAGTAAAAGTCGTAACAAGACTGCGCTCTGTAACGTTACTCTGACGGA2340.3101145038167939No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCCATGGTTGTTG2300.304813401187447No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC2110.2796331636980492No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC1970.261079304495335No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGAAACAGAATCCGAAAAGAGCACA1800.2385496183206107No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCGTAACCGAA1550.20541772688719256No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC1490.19746607294317217No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC1270.16831000848176422No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCG1260.16698473282442747No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG1250.16565945716709077No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGTAAGGATC1220.16168363019508056No Hit
GGAAGTAAAAGTCGTAACAAGTCCTTAATCGGCGGCAGCGCCGCAATGGA1210.16035835453774386No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC1090.14445504664970313No Hit
GGAAGTAAAAGTCGTAACAAGGACGGCATTATTGGTTACGTTCTTGCAAT1060.14047921967769295No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACAGCTCTCCGGCA960.1272264631043257No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTATGTGAACCTGCGGAAGGATC910.12060008481764206No Hit
GGAAGTAAAAGTCGTAACAAGGAATGAACAAGAAGATTTTTGCGGCGCTT890.1179495335029686No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC850.11264843087362172No Hit
GGAAGTAAAAGTCGTAACAAGGAACGTTCAGCAATCGCAGCGGGTTTCCA840.11132315521628498No Hit
GGAAGTAAAAGTCGTAACAAGGTGAGTGAATGGTTGCGGCGTCTGGCCAA830.10999787955894827No Hit
GGAAGTAAAAGTCGTAACAAGGACGGCATCATTGGTTACGTTCTTGCAAT800.10602205258693807No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG790.10469677692960136No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCTGGCA780.10337150127226462No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)