FastQCFastQC Report
Sun 21 May 2023
EGAF00007832401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53543
Sequences flagged as poor quality0
Sequence length247-250
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC3288061.40858749042826No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA721913.482621444446519No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG14162.6446034028724577No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT11182.088041387296192No Hit
GGAAGTAAAAGTCGTAACAAGGCAAAAATATGGAAGCTAAATGAAATGGA8721.6285975757802142No Hit
GGAAGTAAAAGTCGTAACAAGGCAAGAGAAACACGTCAAAGGCCTAAGAA6911.2905515193395962No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT6741.2588013372429636No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA6111.1411388977083838No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC5170.9655790672917095No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAAAGGACCGGAATCTGCTTCATACG3760.7022393216666978No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC2670.498664624694171No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC2600.4855910203014399No Hit
GGAAGTAAAAGTCGTAACAAGGTTCCAGACCGAAGAGATAGAGCTGCGAT2070.3866051584707618No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG1750.3268401098182769No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC1590.29695758549203444No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCGAATCTAATAATGAAGAATCAG1430.267075061165792No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC1370.25586911454345107No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC1340.2502661412322806No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG1320.2465308256915003No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG1270.2371925368395495No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC1200.22411893244681844No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1180.22038361690603814No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCG1070.19983938143174643No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT1060.19797172366135632No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT940.17555983041667444No Hit
GGAAGTAAAAGTCGTAACAAGGAGATCGAGAACAAGGAGGTTTTGCCATG880.16435388379433352No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCGAT820.1531479371719926No Hit
GGAAGTAAAAGTCGTAACAAGGACAAGCAATCGGGATTTTAGCCGGTGAA760.14194199054965168No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA760.14194199054965168No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC660.12326541284575016No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACGGGAACGTGCGGCTGGATC660.12326541284575016No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC640.11953009730496984No Hit
GGAAGTAAAAGTCGTAACAAGGGCACAGCATATCGGGGATTAGTCTATTC640.11953009730496984No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGAAACCTCTCCGGCA640.11953009730496984No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC600.11205946622340922No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC600.11205946622340922No Hit
GGAAGTAAAAGTCGTAACAAGGCATGAGGTTGCAGGATATGGTGTTCATA570.10645649291223876No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA560.10458883514184861No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGAATGAGGCTTTTGCCGAGTTTAA540.10085351960106831No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)