FastQCFastQC Report
Sun 21 May 2023
EGAF00007832409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196347
Sequences flagged as poor quality0
Sequence length247-250
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC17062086.89717693674973No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA28301.441325816029784No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG27601.4056746474354076No Hit
GGAAGTAAAAGTCGTAACAAGGGCTCAAGGAAACTGAACAGGCTACGGGA20331.0354117964623855No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG14540.7405257019460445No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGAAAATGAAGAAATAAAGGTTGGT12840.6539442925025593No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC8710.4436023977957392No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC5660.2882651632059568No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGATAATGAAGAAACAAAGGTTGGT5640.2872465583889746No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCTCGTCTGAC5560.28317213912104594No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC4950.2521046922030894No Hit
GGAAGTAAAAGTCGTAACAAGGGTAACGCCCAATATGGTTACGATCCTGT3870.19710003208605176No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC3360.17112560925300616No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC3340.17010700443602397No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACTTGCGGAAGGATC2630.1339465334331566No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC2620.1334372310246655No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC2580.13140002139070114No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC2520.12834420693975462No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG2010.10236978410670904No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC2010.10236978410670904No Hit
GGACGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC1990.10135117928972687No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)