FastQCFastQC Report
Sun 21 May 2023
EGAF00007832414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85386
Sequences flagged as poor quality0
Sequence length235-238
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG5842868.42807954465603No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT1051412.313494015412363No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT27033.165624341226899No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACACGTGT9941.1641252664371209No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT7080.8291757430960578No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA4200.49188391539596654No Hit
GCTGCGTTCTTCATCGATGCATAGCTTAATTGCATCTGTAGGTCTCCTAT3360.3935071323167732No Hit
GCTGCGTTCTTCATCGACTCGAAACGCCTAGGCATCCACCGTATGCCCTT3340.3911648279577448No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCAAGAGTCG3270.3829667627011454No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT2220.2599957838521537No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGCCGAGAGTCG2160.2529688707750685No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT2110.2471131098774975No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT1960.2295458271847844No Hit
GCTGCGTTCTTCATCGATGCTACCCTTATCTGCAGCAGCTGGAATCTCTG1890.22134776192818495No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG1860.21783430538964235No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC1780.2084650879535287No Hit
GCTGCGTTCTTCATCGATTCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1750.20495163141498607No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCA1600.18738434872227297No Hit
GCTGCGTTCTTCATCGATGCGAGATCCGAGATATCCGTTGCCGAGAGTCG1520.1780151312861593No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG1480.1733305225681025No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATACGTTGCCGAGAGTCG1400.16396130513198884No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG1340.15693439205490362No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCTGTTGCCGAGAGTCG1330.1557632398753894No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCATTGCCGAGAGTCG1230.14405171808024736No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGATTCG1180.1381959571826763No Hit
GCTGCGTTCTTCATCGATGCGTTCAATATTGCTATTTACTTTGCAAATTA1080.12648443538753426No Hit
GCTGCGTTCTTCATCGATGCGATAGCCGAGATATCCGTTGCCGAGAGTCG980.1147729135923922No Hit
GCTGCGTTCTTCATCGTTTCGAAACGCCTAGGCATCCGCCGTATGCCCTT980.1147729135923922No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCAGTTGCCGAGAGTCG970.11360176141287799No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCC950.11125945705384957No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGACGAGAGTCG940.11008830487433538No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG920.10774600051530694No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT920.10774600051530694No Hit
GCTGCGTTCTTCATCGAAGAAAGGCACTGGTAGGCCTAAGTGGACTTGAA910.10657484833579275No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTAG910.10657484833579275No Hit
GCTGCGTTCTTCATCGATGCGAGAGACGAGATATCCGTTGCCGAGAGTCG900.10540369615627855No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCTTTGCCGAGAGTCG890.10423254397676435No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)