FastQCFastQC Report
Sun 21 May 2023
EGAF00007832439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66960
Sequences flagged as poor quality0
Sequence length247-250
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC4386565.50925925925925No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA45336.769713261648745No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG27124.050179211469533No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC26373.938172043010753No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC17542.6194743130227No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC15712.3461768219832737No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC14532.169952210274791No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC9981.490442054958184No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG6660.9946236559139785No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC4510.6735364396654719No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC4230.6317204301075269No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC2800.4181600955794504No Hit
GGAAGTAAAAGTCGTAACAAGGACAAGCAATCGGGATTTTAGCCGGTGAA2110.3151135005973716No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAACCAACATGACTGATGAAGAAAAA1580.23596176821983275No Hit
GGAAGTAAAAGTCGTAACAAGGTTTTCGTAGGTGAACCTGCGGAAGGATC1540.22998805256869773No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC1360.2031063321385902No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAAACTGCGGAAGGATC1330.19862604540023895No Hit
GGAAGTAAAAGTCGTAACAAGGGAAGGTGTGGTGTTCCAAGCAAGGCAGG1270.18966547192353644No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC1260.1881720430107527No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA940.14038231780167262No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCATAGGTGAACCTGCGGAAGGATC930.1388888888888889No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCTGAAGGATC890.13291517323775387No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC810.12096774193548387No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC790.11798088410991638No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC730.10902031063321385No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGTATC710.10603345280764637No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC710.10603345280764637No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCG700.1045400238948626No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT690.10304659498207885No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGAAGGATC680.1015531660692951No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)