FastQCFastQC Report
Sun 21 May 2023
EGAF00007832443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85288
Sequences flagged as poor quality0
Sequence length247-250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC4900157.45356908357565No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG50195.884766907419567No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA43405.088640840446487No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT21602.5325954413282057No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT21522.5232154582121753No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA16991.9920739142669543No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC14691.7223993996810807No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA11591.358925053934903No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA9661.1326329612606698No Hit
GGAAGTAAAAGTCGTAACAAGGCTAAGAAGGACTACAGAAAGAAACAGGG5600.6565988181221274No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT5480.6425288434480818No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC4740.5557639996248006No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGTTGAAAAAGCTGGTAATTGTCAT4500.5276240502767096No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG4420.5182440671606791No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC4310.5053465903761373No Hit
GGAAGTAAAAGTCGTAACAAGGGATGTATAAAGTCCGTATTGGTTCACAG4110.48189663258606136No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA4070.47720664102804616No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC3930.460791670574993No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC3830.44906669167995494No Hit
GGAAGTAAAAGTCGTAACAAGGATTTGCGCAGGTTGAAGATGGTGTACGC3360.3939592908732764No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC3320.3892692993152612No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC3230.37871681830972703No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG3080.36112934996717005No Hit
GGAAGTAAAAGTCGTAACAAGGCGAAAGCCGGTACGACGGATGAGATGAT2760.3236094175030485No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG2700.3165744301660257No Hit
GGAAGTAAAAGTCGTAACAAGGGGGCAGGGCTTCGACCTTCATGCCGCGT2530.29664196604446114No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC2530.29664196604446114No Hit
GGAAGTAAAAGTCGTAACAAGGCAAGAGAAACACGTCAAAGGCCTAAGAA1930.2262920926742332No Hit
GGAAGTAAAAGTCGTAACAAGCCACAGAAGAAATGAACGATCGTAGGGAT1880.22042960322671418No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC1870.2192571053372104No Hit
GGAAGTAAAAGTCGTAACAAGGTTGATGGTAGTAAATGAATTATTGCCAC1720.2016696369946534No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTTCGGAAGGATC1690.19815214332614203No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGTGTTCGAGGAGTTGGGGCAGGGCA1540.18056467498358503No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT1510.17704718131507363No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC1490.17470218553606603No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCGAT1480.17352968764656224No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC1430.16766719819904324No Hit
GGAAGTAAAAGTCGTAACAAGGGCAGAAGGAATTCAGAAGAGCCAGGGTG1390.16297720664102805No Hit
GGAAGTAAAAGTCGTAACAAGGCTTGTGGTAAATGGCAGTAGCCCGTAGG1370.16063221086202045No Hit
GGAAGTAAAAGTCGTAACAAGGATGGAAAGAGGTGGGGGAGGAGAAGAGG1150.13483725729293686No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC1080.1266297720664103No Hit
GGAAGTAAAAGTCGTAACAAGGCCGAGGGACGGTGGGCAATCGATAAGGA1000.1172497889503799No Hit
GGAAGTAAAAGTCGTAACAAGGCATGTTGAAAATGCCATCGTTGCCATTG970.1137322952818685No Hit
GGAAGTAAAAGTCGTAACAAGGCAAAAATAGACAAGTGGGACTATATCAA970.1137322952818685No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGAAACAGAATCCGAAAAGAGCACA930.10904230372385329No Hit
GGAAGTAAAAGTCGTAACAAGGAGAGAGACGATGAGCGTTGATGCAGCGT860.1008348184973267No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)