FastQCFastQC Report
Sun 21 May 2023
EGAF00007832454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61947
Sequences flagged as poor quality0
Sequence length235-238
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT865313.968392335383474No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG832413.437293169967877No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG620810.021469966261481No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG29924.82993526724458No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG27394.421521623323163No Hit
GCTGCGTTCTTCATCGATGCAACCAACCGTTCCGCTGCGTTCTTCATCGA21083.4029089382859543No Hit
GCTGCGTTCTTCATCGATGCAACCAACCGTTCGGCTGCGTTCTTCATCGA19123.0865094354851728No Hit
GCTGCGTTCTTCATCGATGCAACCAACCGTTCTGCTGCGTTCTTCATCGA17072.7555813840863963No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACATGTGT13942.250310749511679No Hit
GCTGCGTTCTTCATCGATGCAACCAACCGTTCAGCTGCGTTCTTCATCGA12722.0533682018499686No Hit
GCTGCGTTCTTCATCGATGCGACTATGGTAAGGCGTAGAAACAGTTTGGC11581.8693399196086975No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT9411.5190404700792612No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT9041.459311992509726No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT8371.3511550196135405No Hit
GCTGCGTTCTTCATCGATGCAACCAACCGTTCGGCTGCGTTCTTCATCGG8201.3237122055951056No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG7541.217169515876475No Hit
GCTGCGTTCTTCATCGATGCACCAACCGTTCCGCTGCGTTCTTCATCGAT7041.1364553569987248No Hit
GCTGCGTTCTTCATCGATGCACCAACCGTTCGGCTGCGTTCTTCATCGAT6961.1235410915782846No Hit
GCTGCGTTCTTCATCGATGCAACCAACCGTTCTGCTGCGTTCTTCATCGG6461.0428269327005344No Hit
GCTGCGTTCTTCATCGATGCAACCAACCGTTCCGCTGCGTTCTTCATCGG6371.0282983841025393No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT6301.0169984018596543No Hit
GCTGCGTTCTTCATCGATGCCCGCATGCCTGTTCGTCGCTACGAGGTGCT6180.997627003728994No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT5910.954041357935009No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT5530.8926985971879187No Hit
GCTGCGTTCTTCATCGATGCACCAACCGTTCTGCTGCGTTCTTCATCGAT4890.7893844738243982No Hit
GCTGCGTTCTTCATCGATGCAACCAACCGTTCAGCTGCGTTCTTCATCGG4890.7893844738243982No Hit
GCTGCGTTCTTCATCGATGCACCAACCGTTCAGCTGCGTTCTTCATCGAT3560.5746848112095824No Hit
GCTGCGTTCTTCATCGATGCTACCTCCAGATTCAAGTCCCGTAGCGACAC3350.5407848644809272No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCATTGCTGAAAGTTG2880.4649135551358419No Hit
GCTGCGTTCTTCATCGATGCACCAACCGTTCGGCTGCGTTCTTCATCGGC2740.4423135906500718No Hit
GCTGCGTTCTTCATCGATGCTCATCAGGGATATTGGCCTAAAATTTTCTT2480.40034222803364167No Hit
GCTGCGTTCTTCATCGAACCAACCGTTCGGCTGCGTTCTTCATCGAACCA2430.3922708121458667No Hit
GCTGCGTTCTTCATCGATGCACCAACCGTTCCGCTGCGTTCTTCATCGGC1880.3034852373803412No Hit
GCTGCGTTCTTCATCGATGCACCAACCGTTCTGCTGCGTTCTTCATCGGC1830.29541382149256623No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT1810.29218525513745625No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC1730.27927098971701614No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT1670.2695852906516861No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT1580.25505674205369105No Hit
GCTGCGTTCTTCATCGATGCGTTGACGTTTTGTCTTTAAGAAACTAGACA1560.25182817569858107No Hit
GCTGCGTTCTTCATCGATGCACACAGGAGCTCAGAATATACCTAGTGAAG1450.23407106074547598No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGCTTTTTTCTTTACGCT1390.22438536168014594No Hit
GCTGCGTTCTTCATCGATGCACCAACCGTTCAGCTGCGTTCTTCATCGGC1380.22277107850259092No Hit
GCTGCGTTCTTCATCGATGCCCACGCCGGTACGCTCGGCACGCTCGGCGC1370.2211567953250359No Hit
GCTGCGTTCTTCATCGAACCAACCGTTCCGCTGCGTTCTTCATCGAACCA1370.2211567953250359No Hit
GCTGCGTTCTTCATCGATGAACCAACCGTTCCGCTGCGTTCTTCATCGAT1270.20501396354948584No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG1170.18887113177393577No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCTGTTGCCGAGAGTCG1110.17918543270860576No Hit
GCTGCGTTCTTCATCGATGCGTTCAATATTGCTATTTACTTCGCAAATCA1110.17918543270860576No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA1100.17757114953105074No Hit
GCTGCGTTCTTCATCGATGAACCAACCGTTCGGCTGCGTTCTTCATCGAT1010.1630426009330557No Hit
GCTGCGTTCTTCATCGATGCATATGTGGTGTGGCCCACATTTCTGTATGC1010.1630426009330557No Hit
GCTGCGTTCTTCATCGATGCCTATCACACCATTTAAGATGTTTTCCTGTG980.15819975140039066No Hit
GCTGCGTTCTTCATCGATGCAACCAACCGTTCGGCTGCGTTCTTCATCTG970.15658546822283564No Hit
GCTGCGTTCTTCATCGATGCGAGATCCGAGATATCCGTTGCCGAGAGTCG900.1452854859799506No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG900.1452854859799506No Hit
GCTGCGTTCTTCATCGATGGGAGAACCTAGAGATCCGTTGTCGAAAGTTG880.1420569196248406No Hit
GCTGCGTTCTTCATCGATGCACCTGAGCCAATTTTTGGCTTGGCGGTAAC880.1420569196248406No Hit
GCTGCGTTCTTCATCGATGTCCCCCTCTGCCTGCTGCGTCTTCTCCACCT880.1420569196248406No Hit
GCTGCGTTCTTCATCGATGCAACCAACCGTTCGGCTGCGTTCTTCTTCTG870.14044263644728558No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCATTGCCGAGAGTCG870.14044263644728558No Hit
GCTGCGTTCTTCATCGAACCAACCGTTCTGCTGCGTTCTTCATCGAACCA850.13721407009217557No Hit
GCTGCGTTCTTCATCGATGAACCAACCGTTCTGCTGCGTTCTTCATCGAT840.13559978691462055No Hit
GCTGCGTTCTTCATCGAAACCAACCGTTCGGCTGCGTTCTTCATCGAAAC810.13075693738195554No Hit
GCTGCGTTCTTCATCGATGCTGGGGCTGGCATTGATAGATTTGGCTACAG810.13075693738195554No Hit
GCTGCGTTCTTCATCGATGCCACCACCTCAATGCCATCTCGCTGATCTGG780.12591408784929053No Hit
GCTGCGTTCTTCATCGATGAACCAACCGTTCAGCTGCGTTCTTCATCGAT760.12268552149418051No Hit
GCTGCGTTCTTCATCGAACCAACCGTTCAGCTGCGTTCTTCATCGAACCA720.11622838878396048No Hit
GCTGCGTTCTTCATCGATGCAACCAACCGTTCTGCTGCGTTCTTCATCTG680.10977125607374044No Hit
GCTGCGTTCTTCATCGAAACCAACCGTTCCGCTGCGTTCTTCATCGAAAC670.10815697289618545No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGACGAGAGTCG650.10492840654107545No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT640.10331412336352043No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACATGCGTGT620.10008555700841042No Hit
GCTGCGTTCTTCATCGATGCAACCAACCGTTCCGCTGCGTTCTTCATCTG620.10008555700841042No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)