FastQCFastQC Report
Sun 21 May 2023
EGAF00007832473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62898
Sequences flagged as poor quality0
Sequence length247-250
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC4031464.09424782981971No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC36185.752170180291901No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC28264.492988648287704No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG26994.29107443797895No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG21753.457979585996375No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC12531.9921142166682566No Hit
GGAAGTAAAAGTCGTAACAAGGCTATGTAAAAAATGACGGTAATAATGGT9751.5501287799294095No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC5290.8410442303411872No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC3390.5389678527139178No Hit
GGAAGTAAAAGTCGTAACAAGGGTTAGTAAATGTTTTAAAATATTCATTT3340.5310184743553054No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGTTGAAAAAGCTGGTAATTGTCAT2850.4531145664409043No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC2320.36885115583961336No Hit
GGAAGTAAAAGTCGTAACAAGGGGGCTGTTGCGGGCAGGTGTCGTTTTGT2130.3386435180768864No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC2020.3211548856879392No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGAAACAGAATCCGAAAAGAGCACA2010.31956501001621673No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC1930.30684600464243694No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTTCGGAAGGATC1580.25120035613215047No Hit
GGAAGTAAAAGTCGTAACAAGGCGTGGGGTACGTTCGGTGCTGGTCTGCT1500.23848135075837068No Hit
GGAAGTAAAAGTCGTAACAAGGAACGTTCAGCGATAGCTGCAGGTTTCCA1460.2321218480714808No Hit
GGAAGTAAAAGTCGTAACAAGGACACTTGGGACCGCAAGGACACCCGCAC1430.2273522210563134No Hit
GGAAGTAAAAGTCGTAACAAGGAAGACAACTGAGGAATGGTGATGTTGCG1400.222582594041146No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT1050.16693694553085947No Hit
GGAAGTAAAAGTCGTAACAAGGCTGAGGTCAAAGTTGCCGATAAGACTAT1010.1605774428439696No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT980.15580781582880218No Hit
GGAAGTAAAAGTCGTAACAAGGTTTTCGTAGGTGAACCTGCGGAAGGATC930.1478584374701898No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCTGAAGGATC900.14308881045502242No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC870.138319183439855No Hit
GGAAGTAAAAGTCGTAACAAGGATTTGCGCAGGTTGAAGGTGGTGTGCAC860.13672930776813252No Hit
GGAAGTAAAAGTCGTAACAAGGCAAAAATAGACAAGTGGGACTATATCAA810.12877992940952018No Hit
GGAAGTAAAAGTCGTAACAAGGTCGTCGTCTTCTGAGTATGCGTTTCCAA780.12401030239435278No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTTAACCTGCGGAAGGATC780.12401030239435278No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG750.11924067537918534No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGTTGAACCTGCGGAAGGATC680.10811154567712804No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGAAGGATC650.10334191866196063No Hit
GGAAGTAAAAGTCGTAACAAGGACGAGGTGGACATCCGCGTGGCGATCAG650.10334191866196063No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC640.10175204299023816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)