FastQCFastQC Report
Sun 21 May 2023
EGAF00007832481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157117
Sequences flagged as poor quality0
Sequence length247-250
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC8319552.95098557126218No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCTATGGTAGTTG1638410.42789768134575No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC61983.9448309221790128No Hit
GGAAGTAAAAGTCGTAACAAGTCCGCAGGATTTAGTATCCATGGTTGTTG40682.5891533061349183No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCCATGGTTGTTG32742.083797424848998No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC27291.7369221662837249No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA26781.7044622797023874No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT26661.696824659330308No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC20661.3149436407263377No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC17971.143733650718891No Hit
GGAAGTAAAAGTCGTAACAAGGAAGACAACTGAGGAATGGTGATGTTGCG11350.7223915935258438No Hit
GGAAGTAAAAGTCGTAACAACCAGAGACTCAAGGGGAAGCCCCATCTGAC10660.6784752763863872No Hit
GGAAGTAAAAGTCGTAACAAGGATGTGCATGTACCCATCGTAAAGCGAAG9150.5823685533710546No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC8350.5314510842238587No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC7210.45889369068910435No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC7020.4468007917666453No Hit
GGAAGTAAAAGTCGTAACAAGGACAAGGTGACCTTCTCGAACAAGCTGGA6670.4245243990147469No Hit
GGAAGTAAAAGTCGTAACAAGGATATGAAGATTGTCAATATGATGCAGAC6480.4124315000922879No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA5760.36660577785981147No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGAGCCAATGATCGCTGAGAAGGT5170.3290541443637544No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGAGAACATCTGTGCGCGCTGGAAGG4280.2724084599374988No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC3790.24122151008484122No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGAAACAGAATCCGAAAAGAGCACA3550.22594626934068243No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC3550.22594626934068243No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGATAGCTCTCCGGCAACTCGAGA3530.22467333261200254No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTATGTGAACCTGCGGAAGGATC3230.205579281681804No Hit
GGAAGTAAAAGTCGTAACAAGGAGAAAGGCCAGACGGGTGTTAAGCTATT3220.20494281331746406No Hit
GGAAGTAAAAGTCGTAACAAGGCATGAAGACTGCAACACTTTCCAAGCAT3210.2043063449531241No Hit
GGAAGTAAAAGTCGTAACAAGGCGCGTAAGCGTGCCGCTGATGTCGTCTA3170.20176047149576432No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTTCGGAAGGATC3160.20112400313142434No Hit
GGAAGTAAAAGTCGTAACAGGCGTTAAGTTTACAGCATGAAGAGAACAAA3120.19857812967406455No Hit
GGAAGTAAAAGTCGTAACAACCAGAGATTCCAGGGGAAGCCCCATTTGGC2880.18330288892990573No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC2860.18202995220122584No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATA2780.17693820528650622No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA2640.16802764818574695No Hit
GGAAGTAAAAGTCGTAACAAGGATATGAAGGTTGTAAATATGATGCAGGC2530.1610264961780075No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC2490.15848062272064767No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGTAAGGATC2370.15084300234856826No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTAAACCAGCGGAGGGATC2250.14320538197648885No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGTATC2170.13811363506176924No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCTTAGGTGAACCTGCGGAAGGATC2140.1362042299687494No Hit
GGAAGTAAAAGTCGTAACAAGGCGATCGGACAGTTTGACCTGTTCGGTGC2050.13047601468968986No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCCTCCTCATG2040.1298395463253499No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCGTCCTCATG1840.1171101790385509No Hit
GGAAGTAAAAGTCGTAACAAGGAAACCACTTAACAGATAGAAAAATCCGA1700.10819962193779158No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC1680.10692668520911168No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTTAACCTGCGGAAGGATC1640.10438081175175187No Hit
GGAAGTAAAAGTCGTAACAAGGTCATTGAGGAAGAGGAGAGAGAGCTCTC1620.10310787502307198No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)