FastQCFastQC Report
Sun 21 May 2023
EGAF00007832491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127352
Sequences flagged as poor quality0
Sequence length247-250
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC7800861.25384760349269No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA1734013.615805012877694No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA78576.169514416734719No Hit
GGAAGTAAAAGTCGTAACAAGAGATGGAGATTAGTCAATATTATCGAAAG46443.646585840819147No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC23121.8154406683836926No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT14861.16684465104592No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA9560.7506752936742258No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC8940.7019913311137634No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA4700.36905584521640805No Hit
GGAAGTAAAAGTCGTAACAAGGGATGGAGATTAGTCAATATTATCGAAAG4680.3674853948112319No Hit
GGAAGTAAAAGTCGTAACAAGGAACGTTCAGCTATCGCAGCGGGCTTCCA4020.3156605314404171No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG2770.21750738111690435No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC2760.21672215591431623No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC2600.2041585526729066No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTTAAGGTTTCGGATAAAGCA2450.19238017463408505No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC2170.1703938689616182No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACTGAATCCGAAAAGAGCACA1960.15390413970726805No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC1900.14919278849173942No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTAGGATAAAGCA1800.14134053646585842No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTTCGGAAGGATC1740.1366291852503298No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC1710.1342735096425655No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC1710.1342735096425655No Hit
GGAAGTAAAAGTCGTAACAAGGGGAGTCCCAAGCAGGATGTTTACCGCGC1670.1311326088322131No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTTAACCTGCGGAAGGATC1650.12956215842703686No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC1630.12799170802186066No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTATGTGAACCTGCGGAAGGATC1560.12249513160374395No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC1550.12170990640115585No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGTTGAACCTGCGGAAGGATC1550.12170990640115585No Hit
GGAAGTAAAAGTCGTAACAAGGTTACCAACGTAGCCGCCGGTACCGAAGA1470.11542810478045104No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC1420.11150197876751052No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGTAAGGATC1320.1036497267416295No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC1320.1036497267416295No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)