FastQCFastQC Report
Sun 21 May 2023
EGAF00007832500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85112
Sequences flagged as poor quality0
Sequence length235-238
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG4423651.973869724598174No Hit
GCTGCGTTCTTCATCGATTTCGCCCAACAATGCTTCAGGAGAAACGACAA54846.443274743866905No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT46875.506861547137889No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT41114.830106212989942No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT22892.6893974997650156No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACATGTGT22562.650625058746123No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG18382.1595074725068146No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA18012.11603534166745No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG10941.2853651658990506No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT9181.0785788137982895No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT8220.9657862581069648No Hit
GCTGCGTTCTTCATCGATGCGGAGCTTGACGTCGCGTGGGCAGATCATCC7690.903515367985713No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCA6160.7237522323526647No Hit
GCTGCGTTCTTCATCGGCGCGGTGCTTGTCATTTACTACAAACAAATTTC5140.6039101419306325No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATTAAAATAA4190.49229250869442615No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT4010.4711439045023028No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC3480.40887301438105084No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG3350.39359902246451733No Hit
GCTGCGTTCTTCATCGATGCCAGTTGGCGGGTGAATACTGTAATGTCTTC3200.3759751856377479No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT2830.33250305479838327No Hit
GCTGCGTTCTTCATCGATGCCGGTCCCTGTCCGTAAGGCTTTGTTGGGAG2730.32075383024720366No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCTCCTGTCCGAGGT2710.31840398533696773No Hit
GCTGCGTTCTTCATCGATTTCACCCAACAATGCTTCAGGAGAAACGACAA2580.3031299934204343No Hit
GCTGCGTTCTTCATCGATGCATATGTGGTGTGGCCCACATTTCTGTATGC2390.28080646677319293No Hit
GCTGCGTTCTTCATCGATGCTCATCAGGGATATTGGCCTAAAATTTTCTT1900.22323526647241282No Hit
GCTGCGTTCTTCATCGATGCCTATCACACCATTTAAGATGTTTTCCTGTG1720.2020866622802895No Hit
GCTGCGTTCTTCATCGATGCGTACATAAACTATGTAGGAACGCCCGCGCT1410.16566406617163268No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT1390.16331422126139675No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT1370.16096437635116084No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG1350.1586145314409249No Hit
TCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1320.155089764075571No Hit
GCTGCGTTCTTCATCGGCGCAGTCCTTGTCATTTACTACAAACAAATTTC1310.15391484162045305No Hit
GCTGCGTTCTTCATCGATGCGAGAGTCGAGATATCCGTTGCCGAGAGTCG1290.15156499671021714No Hit
GCTGCGTTCTTCATCGATGCCCACGCCGGTACGCTCGGCACGCTCGGCGC1180.13864084970391954No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCTAGAGTCG1090.12806654760785788No Hit
GCTGCGTTCTTCATCGATGCTGTCCATGGTCACGCGATGCTCTCCTCGAT1070.12571670269762197No Hit
GCTGCGTTCTTCATCGATGCGGTGCTTGTCATTTACTACAAACAAATTTC1060.12454178024250401No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGTCAAACT1060.12454178024250401No Hit
GCTGCGTTCTTCATCGATTCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1000.11749224551179623No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCAGAGAGTCG950.11161763323620641No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCATTGCCGAGAGTCG930.10926778832597048No Hit
GCTGCGTTCTTCATCGATGCGGGATCGATGGGGCGCGTGCGAGCCTGTGG920.10809286587085254No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTTCCGAGAGTCG910.10691794341573457No Hit
GCTGCGTTCTTCATCGATGCACACCCGTTTGACCCGCAAGCGCAGGATAT910.10691794341573457No Hit
GCTGCGTTCTTCATCGAGGTCGATGAAGGAGTCATAGCGCTCGGAGCGCA910.10691794341573457No Hit
GCTGCGTTCTTCATCGATGCTTGCAAGAAATCGCGTCTTAAACAGTTTGA900.10574302096061661No Hit
GCTGCGTTCTTCATCGATCGTCTTCCTTATTCTCAGACAAAATAAACTTC890.10456809850549864No Hit
GCTGCGTTCTTCATCGATGCCAGTTGGCGCGTAAACACCGTGATGTCTTC890.10456809850549864No Hit
GCTGCGTTCTTCATCGATGCATTCAATATTGCTATTTACTTTGCAAATTA880.10339317605038068No Hit
GCTGCGTTCTTCATCGATGCGAGAGCAGAGATATCCGTTGCCGAGAGTCG880.10339317605038068No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)