FastQCFastQC Report
Sun 21 May 2023
EGAF00007832513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111912
Sequences flagged as poor quality0
Sequence length247-250
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC9085081.17985560082921No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG25322.262491957966974No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC15011.3412323968832656No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG11551.0320609049967833No Hit
GGAAGTAAAAGTCGTAACAAGGTGCCCAAATGAAGCACGCCGGGGCGGAC9200.8220744870970048No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGAAACAGAATCCGAAAAGAGCACA7470.6674887411537637No Hit
GGAAGTAAAAGTCGTAACAAGGCTACAGAAAAGAAAGTTTTGGTGGGCAA6350.5674101079419543No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC6340.566516548716849No Hit
GGAAGTAAAAGTCGTAACAAGGCGGTGCAGACCATCACCGAGCAGCAGGC4700.4199728357995568No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT4570.4083565658731861No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACTGAATCCGAAAAGAGCACA4530.4047823289727643No Hit
GGAAGTAAAAGTCGTAACAAGGCGGAGTTGCACAAACTCTTTTTTAACTT3710.33151047251411825No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC3040.2716420044320538No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGATAGTCCTGAAGTATCTCTTCA2750.245728786903996No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA2340.20909285867467295No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC2200.19658302952319678No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC2170.1939023518478805No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC2080.18586031882193152No Hit
GGAAGTAAAAGTCGTAACAAGGGCGGGTGTGGTGTTCCAAGCAAGGCAGG2050.1831796411466152No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC2050.1831796411466152No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC1970.17603116734577168No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC1600.1429694760168704No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCATAGGTGAACCTGCGGAAGGATC1380.12331117306455072No Hit
GGAAGTAAAAGTCGTAACAAGTCCTTAATCGGCGGCAGCGCCGCAATGGA1360.12152405461433984No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC1360.12152405461433984No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTATGTGAACCTGCGGAAGGATC1320.11794981771391809No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGTTGAACCTGCGGAAGGATC1290.11526914003860175No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGTATC1230.1099077846879691No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC1160.10365287011223105No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1130.10097219243691472No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)