FastQCFastQC Report
Sun 21 May 2023
EGAF00007832527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137981
Sequences flagged as poor quality0
Sequence length247-250
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC5746641.647763097817815No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA2913121.112327059522688No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC77255.59859690826998No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC49243.5686072720157123No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC43163.127966893992651No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG32282.339452533319805No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC32042.3220588341873154No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG24551.7792304737608802No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC12610.9138939419195395No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT10800.7827164609620164No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC7860.5696436465890231No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC7480.5421036229625819No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT7470.541378885498728No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC6420.4652814517940876No Hit
GGAAGTAAAAGTCGTAACAAGGAAGACAACTGAGGAATGGTGATGTTGCG6010.4355672157760851No Hit
GGAAGTAAAAGTCGTAACAAGGTTAGTCGCACCGTAACTTCCGACAATCA5560.40295402990266777No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC4930.35729556967988346No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG4540.32903080858958844No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCTTAGGTGAACCTGCGGAAGGATC3940.28554656075836526No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC3940.28554656075836526No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGAAAATGAAGAAATAAAGGTTGGT3780.27395076133670576No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA3770.27322602387285205No Hit
GGAAGTAAAAGTCGTAACAAGGGCTCTAATTAGACTCAGATGAAGAACGC3310.239888100535581No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGTGAACGTGCGGATGGACT3200.23191598843319006No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG2910.21089860198143223No Hit
GGAAGTAAAAGTCGTAACAAGGTTCCAGGGGTGCTTTCGCCGGAAGTCCC2450.17756067864416117No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC2310.16741435415020908No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCG2280.16524014175864793No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATA2190.15871750458396444No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGCATC2120.15364434233698843No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGATAATGAAGAAACAAAGGTTGGT2050.1485711800900124No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCTATGGTAGTTG1920.13914959305991403No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC1800.13045274349366942No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC1770.12827853110210827No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1620.11740746914430247No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGGGAACCTGCGGAAGGATC1600.11595799421659503No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTATGTGAACCTGCGGAAGGATC1590.11523325675274132No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGTGGCATAGATGGTGATAACTTGTA1560.11305904436118017No Hit
GGAAGTAAAAGTCGTAACAAACAGGGAGTTGTGGTGGGAAGGAGCTTGCA1530.110884831969619No Hit
GGAAGTAAAAGTCGTAACAAGGATGGCATCATTGGTTACGTTCTTGCAAT1530.110884831969619No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTTCGAGAACGAGCGGCTGGATC1520.11016009450576529No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCCTAGGAGCT1510.10943535704191157No Hit
GGAAGTAAAAGTCGTAACAAGGGAACCGCTTTTACGCAGGAGGAGCGCCA1430.10363745733108182No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACAACTCTCCGGCA1430.10363745733108182No Hit
GGAAGTAAAAGTCGTAACAAGGCAAAAATAGACAAGTGGGACTATATCAA1380.10001377001181322No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)