FastQCFastQC Report
Sun 21 May 2023
EGAF00007832531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140177
Sequences flagged as poor quality0
Sequence length247-250
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC7564953.9667705829059No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC91586.533168779471668No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT64474.599185315707998No Hit
GGAAGTAAAAGTCGTAACAAGCCACAGAAGAAATGAACGATCGTAGGGAT35812.5546273639755452No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC29312.090927898300006No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC26501.8904670523695044No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC25711.8341097326950926No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG25611.8269758947616228No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG22841.6293685840045085No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA22511.6058269188240584No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCG19661.4025125377201682No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCGTCGTTACC18721.3354544611455517No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC15141.0800630631273318No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC14941.0657953872603922No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC7590.5414582991503599No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCTATGGTAGTTG6830.4872411308559892No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC6390.455852243948722No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT6280.44800502222190514No Hit
GGAAGTAAAAGTCGTAACAAGGCTGAGTTGCACAAACTCTTTTTTGATTT5940.4237499732481077No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC5400.38522724840737066No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC4900.34955805874002155No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAACACA4270.3046148797591616No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC4260.3039014959658146No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGAAAATGAAGAAATAAAGGTTGGT3890.27750629561197626No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA3720.2653787711250776No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC3690.26323861974503665No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC3440.24540402491136207No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC3250.2318497328377694No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTACGGCG2950.2104482190373599No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTTCGGAAGGATC2920.20830806765731896No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA2580.18405301868352153No Hit
GGAAGTAAAAGTCGTAACAAGCGGTAAGAAGAACACAATCAACACTTGAC2520.17977271592343966No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC2520.17977271592343966No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG2370.1690719590232349No Hit
GGAAGTAAAAGTCGTAACAAGAGATGGAGATTAGTCAATATTATCGAAAG2360.16835857522988792No Hit
GGAAGTAAAAGTCGTAACAAGGCTATGTAAAAAATGACGGTAATAATGGT2170.15480428315629524No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCGATGGTAGTTG2150.1533775155696013No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA2130.1519507479829073No Hit
GGAAGTAAAAGTCGTAACAAGGGCAAGCCCAGCTCGCGCTCGATTGCGGC2080.14838382901617242No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGATAATGAAGAAACAAAGGTTGGT2040.14553029384278449No Hit
GGAAGTAAAAGTCGTAACAAGGCAAAAATAGACAAGTGGGACTATATCAA1980.1412499910827026No Hit
GGAAGTAAAAGTCGTAACAAGGCCCACCAAATCGGGATATCGGCACTTAC1920.1369696883226207No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCGAT1880.13411615314923275No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGTGGCATAGATGGTGATAACTTGTA1840.13126261797584482No Hit
GGAAGTAAAAGTCGTAACAAGGACAATTGATCGCACAAATTGATTCTGTA1820.12983585038915085No Hit
GGAAGTAAAAGTCGTAACAGGCGTTAAGTTTACAGCATGAAGAGAACAAA1740.124128780042375No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGTTGAACCTGCGGAAGGATC1690.12056186107564008No Hit
GGAAGTAAAAGTCGTAACAAGGGCGGCGAAGACGTCGAGAAGGAAGCGGC1560.1112878717621293No Hit
GGAAGTAAAAGTCGTAACAAGGTGTACATAAAAGGCGACGGCGGAAAATG1530.10914772038208835No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGAGAACCTGCGGCTGGATC1520.10843433658874137No Hit
GGAAGTAAAAGTCGTAACAAGGTCAAGCTCCTAAGGACAAAAAACAAGAT1510.1077209527953944No Hit
GGAAGTAAAAGTCGTAACAAGGGAAGGTTGTCATGCTACAGAGTGGTCCT1470.10486741762200646No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC1410.10058711486192456No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)