FastQCFastQC Report
Sun 21 May 2023
EGAF00007832535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74292
Sequences flagged as poor quality0
Sequence length247-250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC3334444.8823561083293No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC1264117.01529101383729No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA881011.85861196360308No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG67429.07500134603995No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC26543.572390028536047No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC10331.3904592688310988No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG10011.3473859904161956No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC7410.9974156032951058No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT6210.8358908092392182No Hit
GGAAGTAAAAGTCGTAACAAGGGACAGGCCGACGGCGACAACGACGAATG5030.6770580950842621No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC1550.20863619232218814No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC1400.18844559306520217No Hit
GGAAGTAAAAGTCGTAACAAGTCCTTAATCGGCGGCAGCGCCGCAATGGA1380.18575351316427072No Hit
GGAAGTAAAAGTCGTAACAAGGTGGAATATATTACCTGGTCATAAGGAGA1330.17902331341194205No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC1270.1709470737091477No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC1270.1709470737091477No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG1170.1574866742044904No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCAGAGCGTCATCGAGAACGGCGC1080.14537231465029882No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG970.1305658751951758No Hit
GGAAGTAAAAGTCGTAACAAGGTGTACATAAAAGGCGACGGCGGAAAATG950.12787379529424434No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTTAACCTGCAGAAGGATC940.1265277553437786No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCG840.11306735583912131No Hit
GGAAGTAAAAGTCGTAACAAGTCCTTAATCGGCGGCAGCGCAGCAATGGA840.11306735583912131No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCCGCGGAAGGATC780.10499111613632693No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT760.10229903623539546No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC750.10095299628492974No Hit
GGAAGTAAAAGTCGTAACAAGGATGGAAAGAGGTGGGGGAGGAGAAGAGG750.10095299628492974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)