FastQCFastQC Report
Sun 21 May 2023
EGAF00007832547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191095
Sequences flagged as poor quality0
Sequence length247-250
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCG5764730.166671027499408No Hit
GGAAGTAAAAGTCGTAACAAGGCGGAGTTGCACAAACTCTTTTTTGATTT150497.875140636856013No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC143897.529762683482037No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC130256.815981579842487No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT107585.629660639995813No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC89074.661032470760616No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG71443.7384546953086164No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC68313.5746618174206546No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG65463.425521337554619No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC49692.600277348962558No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCGAATCTAATAATGAAGAATCAG45182.364269080823674No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA24351.2742353279782306No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA12870.6734870090792539No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC12010.6284832151547659No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC11130.5824328213715692No Hit
GGAAGTAAAAGTCGTAACAAGGCCGTGAAGTAAGCGGGAAGCATATTAAT9640.5044611318977472No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC8800.46050393783196836No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC8640.45213113896229623No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC8430.4411418404458516No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG6210.32496925612915045No Hit
GGAAGTAAAAGTCGTAACAAGGTCGACAACTTTATGGAGACCAGCGTCAA6050.31659645725947827No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACACATGGCTCAAGCAGAGCAAAA5710.29880425966142493No Hit
GGAAGTAAAAGTCGTAACAAGGCTGAGTTGCACAAACTCTTTTTTGATTT5420.28362856171014417No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGAAACCTCTCCGGCG5200.27211596326434495No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCTACGGTCTT4640.24281116722049242No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC4640.24281116722049242No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC4600.24071796750307436No Hit
GGAAGTAAAAGTCGTAACAAGGGTTGATGAGACAACTTACACATTTGTAA4250.22240246997566657No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT4150.21716947068212145No Hit
GGAAGTAAAAGTCGTAACAAGGTCGACAAGTTTATGGAGACCAGCGTCAA3880.2030403725895497No Hit
GGAAGTAAAAGTCGTAACAAGGTATGAGAAGAAGAGCCCAGGTGAGGCAA3860.20199377273084068No Hit
GGAAGTAAAAGTCGTAACAAGGGTGTAGAGTATAAGCTTGGAGCCCGTGG3460.1810617755566603No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC3450.1805384756273058No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGTAAGGATC3360.17582877626311522No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTTAACCTGCAGAAGGATC3320.17373557654569716No Hit
GGAAGTAAAAGTCGTAACAAGGTGCTTGGGGATACCATTTACGGGCATTA3310.17321227661634264No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACATCTCCGGCG3030.1585598785944164No Hit
GGAAGTAAAAGTCGTAACAAGGACAAGCAATCGGGATTTTAGCCGGTGAA2990.15646667887699836No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT2870.15018707972474424No Hit
GGAAGTAAAAGTCGTAACAAGGTACCCGAATGCCGGATTCCTCGCTAACA2800.14652398021926266No Hit
GGAAGTAAAAGTCGTAACAAAGGTTGATGAGACAACTTACACATTTGTAA2710.14181428085507208No Hit
GGAAGTAAAAGTCGTAACAAGGTTGGCGGGATTTACACAGTTCTTTCGAC2500.13082498233862738No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAGTGGAACTTTGCAGGGGATGAAAA2490.13030168240927287No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGGG2410.1261152829744368No Hit
GGAAGTAAAAGTCGTAACAAACAGGGAGTTGTGGTGGGAAGGAGCTTGCA2340.12245218346895524No Hit
GGAAGTAAAAGTCGTAACAAGGTCAAGCTCCTAAGGACAAAAAACAAGAT2170.11355608466992856No Hit
GGAAGTAAAAGTCGTAACAAGGGCACAGCATATCGGGGATTAGTCTATTC2170.11355608466992856No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAGATGAAGGACACCTCTCCGGCG2150.11250948481121956No Hit
GGAAGTAAAAGTCGTAACAAGGGCTAGAAAAAGAGCTCGAAGCGTTAGAT2130.11146288495251053No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA2110.1104162850938015No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA2070.10832308537638347No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC2050.10727648551767445No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGTGAACGTGCGGATGGACT1990.1041366859415474No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTACGGAG1960.10256678615348387No Hit
GGAAGTAAAAGTCGTAACAAGGAAGACAACTGAGGAATGGTGATGTTGCG1920.10047358643606583No Hit
GGAAGTAAAAGTCGTAACAAGGTGCGAGACCTCGAAGACCTGATGGAAAA1920.10047358643606583No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)