FastQCFastQC Report
Sun 21 May 2023
EGAF00007832553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147290
Sequences flagged as poor quality0
Sequence length247-250
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG7128448.397039853350535No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC3412723.16993685925725No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC64044.347885124584154No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG55363.758571525561817No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC24581.6688166202729309No Hit
GGAAGTAAAAGTCGTAACAAGGCGGAGTTGCACAAACTCTTTTTTGATTT19941.3537918392287325No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACATCTCTCCGGCA18411.2499151334102792No Hit
GGAAGTAAAAGTCGTAACAAGGCAAAAATAGACAAGTGGGACTATATCAA16611.1277072442120986No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC13630.9253852943173331No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG13460.9138434381152827No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC12820.8703917441781519No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT11260.7644782402063955No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC8930.6062869169665286No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGGCGAGGAAAATGGTGAGGGAGA6800.4616742480820151No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG6630.45013239187996473No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGATAGTCCTGAAGTATCTCTTCA6460.43859053567791434No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC5560.3774865910788241No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGAAAATGAAGAAATAAAGGTTGGT4100.27836241428474434No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCGTAACCGAA3640.2471315092674316No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC3600.24441577839636092No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGATAATGAAGAAACAAAGGTTGGT3400.23083712404100756No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC3350.2274424604521692No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC2800.19010116097494736No Hit
GGAAGTAAAAGTCGTAACAAGGAAATGCGGATCCTCATGTTGGGCCTGGA2730.18534863195057372No Hit
GGAAGTAAAAGTCGTAACAAGGTTTTCGTAGGTGAACCTGCGGAAGGATC2500.16973317944191732No Hit
GGAAGTAAAAGTCGTAACAAACAGGGAGTTGTGGTGGGAAGGAGCTTGCA2450.16633851585307896No Hit
GGAAGTAAAAGTCGTAACTAAGAATGGGTTGCCTGCCAGCCCTGGCAGGT2380.16158598682870529No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG2300.1561545250865639No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA1990.13510761083576617No Hit
GGAAGTAAAAGTCGTAACAAAGTTTTGGTAGGTGAACCTGTGGAGGGATC1520.10319777310068572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)