FastQCFastQC Report
Sun 21 May 2023
EGAF00007832555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79299
Sequences flagged as poor quality0
Sequence length247-250
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC3556744.85176357835534No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC30153.802065599818409No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG27853.512024111275048No Hit
GGAAGTAAAAGTCGTAACAAGGGCGGGTGTGGTGTTCCAAGCAAGGCAGG20822.6255059962925134No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGTGGCTGGAAC18512.334203457798963No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT15601.967237922294102No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC13831.744032081110733No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG13131.6557585845975358No Hit
GGAAGTAAAAGTCGTAACAAGGCTACGGAAAAGAAAGTTTTGGTGGGCAA13011.6406259851952738No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGTTGAAAAAGCTGGTAATTGTCAT12721.6040555366398062No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC12481.5737903378352815No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC11621.4653400421190683No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC11591.4615568922685027No Hit
GGAAGTAAAAGTCGTAACATGAGTGCAGGCTTCCAGGAACTGGCAACAGG11311.426247493663224No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC9631.2143911020315514No Hit
GGAAGTAAAAGTCGTAACAAGGACATCGCAGAAAGAGCCTGCGTATCTGT8561.0794587573613792No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC7450.9394822128904526No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA6600.8322929671244279No Hit
GGAAGTAAAAGTCGTAACAAGGCGAGGACAAGCGGTGAGATAACCGATGC4230.5334241289297469No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA3470.4375843327154188No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC3350.42245173331315655No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC3110.3921865345086319No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAATTACAGCAACTAGGACCTGTTGT2880.3631823856542958No Hit
GGAAGTAAAAGTCGTAACAAGAGTGCAGGCTTCCAGGAACTGGCAACAGG2880.3631823856542958No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAACCAACATGACTGATGAAGAAAAA2820.3556160859531646No Hit
GGAAGTAAAAGTCGTAACAAGGAACTGGAGGCGGAACGCGACAGCTATCT2680.3379613866505252No Hit
GGAAGTAAAAGTCGTAACAAGGCGGCCGATGGCAGCATCTACAACGTCGG2670.33670033670033667No Hit
GGAAGTAAAAGTCGTAACAAGGTGGAATCGGGTGTGCGTCTGCGCTATCA2610.32913403699920557No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGCAACATCATCAGATATTCATAACA2430.30643513789581206No Hit
GGAAGTAAAAGTCGTAACAAGGGTGTAGAGTATAAACTCGGCGCTCGTGG2410.30391303799543495No Hit
GGAAGTAAAAGTCGTAACAAGGGCGGTGGGGTTGCTGTCTATGCCTTGTG2330.2938246383939268No Hit
GGAAGTAAAAGTCGTAACAAGGCGAAACCGAAGTCATGTTGCGCCCGCTG2000.2522099900377054No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCTATGGTAGTTG1960.2471657902369513No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGCGGTGTGGGCGACATTGTATTGCT1950.24590474028676276No Hit
GGAAGTAAAAGTCGTAACAAGGTGTACATAAAAGGCGACGGCGGAAAATG1850.23329424078487748No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT1770.22320584118336928No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT1700.21437849153204958No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGTATC1660.2093342917312955No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTTCGGAAGGATC1660.2093342917312955No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG1640.20681219183091842No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC1630.2055511418807299No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC1590.20050694207997577No Hit
GGAAGTAAAAGTCGTAACAAGGTTGATAGTAGTAAATGAGTTGTTGCCAC1530.19294064237884462No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACGGGAACGTGCGGCTGGATC1510.1904185424784676No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC1500.18915749252827904No Hit
GGAAGTAAAAGTCGTAACAAGGGGTAAGCTGAGGGGATGCGTTGGCATCG1470.18537434267771347No Hit
GGAAGTAAAAGTCGTAACAAGGCATTAAAGAGGCGCACGAGCGCAACAAA1400.17654699302639376No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAATTGAACGTTACCGTAAGTCGCT1400.17654699302639376No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1360.17150279322563966No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC1340.1689806933252626No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCATAGGTGAACCTGCGGAAGGATC1330.16771964337507408No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC1320.16645859342488556No Hit
GGAAGTAAAAGTCGTAACAAGGGTGTAGAGTATAAGCTTGGAGCCCGTGG1290.16267544357431998No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC1240.15637019382337736No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG1230.1551091438731888No Hit
GGAAGTAAAAGTCGTAACAAGGAAGCTTATGATTGAAGTAGAGTCCAAAT1180.14880389412224618No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTATGTGAACCTGCGGAAGGATC1160.14628179422186913No Hit
GGAAGTAAAAGTCGTAACAAGGGATACCGTGGCTCAACCTTCTTTAGCGG1150.1450207442716806No Hit
GGAAGTAAAAGTCGTAACAAGGCGATCGGACAGTTTGACCTGTTCGGTGC1040.1311491948196068No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTATAGGTGAACCTGCGGAAGGATC1010.12736604496904122No Hit
GGAAGTAAAAGTCGTAACAAGGTTGGCGGGATTTACACAGTTCTTTCGAC990.12484394506866417No Hit
GGAAGTAAAAGTCGTAACAAGGATACCCGTGTTGGCAATATGGCGCTCGT980.12358289511847564No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCCGACGTCAT930.117277645367533No Hit
GGAAGTAAAAGTCGTAACAAGGAAGACAACTGAGGAATGGTGATGTTGCG920.11601659541734448No Hit
GGAAGTAAAAGTCGTAACAAGGTTGGCGGGATTTACACGGTTCTATCTAC910.11475554546715595No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCGAT880.11097239561659038No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC870.10971134566640185No Hit
GGAAGTAAAAGTCGTAACAAGGGAACCGCTTTTACGCAGGAGGAGCGCCA830.10466714586564775No Hit
GGAAGTAAAAGTCGTAACAAGGACATCGAAGAAAGAGCCTGCGTATCTGT800.10088399601508216No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)