FastQCFastQC Report
Sun 21 May 2023
EGAF00007832581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171714
Sequences flagged as poor quality0
Sequence length247-250
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC5570932.44289923943301No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC2332013.580721432148806No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG1723310.035873603783035No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC146608.53745180940401No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC75444.393351736026183No Hit
GGAAGTAAAAGTCGTAACAAGGATAGAGACTTGGTATGAGTGCTGGAAGG72264.208160080133245No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC60073.498258732543648No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA58973.434198725788229No Hit
GGAAGTAAAAGTCGTAACAAGGTGGCTGAACTGACGTGGGAACAGGTCCG52453.0544975948379283No Hit
GGAAGTAAAAGTCGTAACAAGGGGAATCTTGAATGTCTTACCGAGAAGAG47232.7505037445985767No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG16730.9742944663801437No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT14470.8426802706826467No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC9600.559069149865474No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC7070.4117311343280105No Hit
GGAAGTAAAAGTCGTAACAAGGACGAGGAGGGCAAGGAAAAGTGGAGGAG6890.4012485877680329No Hit
GGAAGTAAAAGTCGTAACAAGGACAGAGCAACGTATCAGGCGGCGGTGGC6810.3965896781858206No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA6630.386107131625843No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC6420.3738774939725357No Hit
GGAAGTAAAAGTCGTAACAAGGAACCAAATGTTAATCGCCAAAACATTGG6350.3698009480881No Hit
GGAAGTAAAAGTCGTAACAAGGACAGGGGGCTTCTTCTTTAGAACTGGGT6090.35465949194591007No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGAAAATGAAGAAATAAAGGTTGGT5740.33427676252373134No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC5100.29700548586603304No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC4930.28710530300383197No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCCAAGGTACG4360.25391057223056945No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG3720.21663929557287115No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG3580.20848620380399968No Hit
GGAAGTAAAAGTCGTAACAAGGCGTAGAAGCATCGATGAAGAACGCAGCC3420.1991683846395751No Hit
GGAAGTAAAAGTCGTAACAAGGCCAATGAACCGATGATCGCTGAGAAGGT3250.189268201777374No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT3130.18227983740405557No Hit
GGAAGTAAAAGTCGTAACAAGGCGTGGGGTACGTTCGGTGCTGGCCTGCT2900.16888547235519524No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG2560.14908510663079305No Hit
GGAAGTAAAAGTCGTAACAAGGCCAGCACGCCCATGCCCGCCACCAAAGT2490.14500856074635732No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC2170.1263729224175082No Hit
GGAAGTAAAAGTCGTAACAAGGCAACGACGAGCACGATGCCGAGGATTTT1890.11006673887976519No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)