FastQCFastQC Report
Sun 21 May 2023
EGAF00007832614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65539
Sequences flagged as poor quality0
Sequence length235-238
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT2360836.02130029448115No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT1970030.058438487007738No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG684910.450266253681015No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG20343.1034956285570425No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT6921.0558598696959063No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT6691.0207662613100597No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT5660.863607928103877No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA5440.8300401287782847No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG5200.7934207113321838No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG4460.6805108408733731No Hit
GCTGCGTTCTTCATCGATGCGGAGCTTGACGTCGCGTGGGCAGATCATCC4300.6560978959093059No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG3590.5477654526312578No Hit
GCTGCGTTCTTCATCGATGCCAAGCCGCTCTTGCGTTGCGTAGATAACAC3230.4928363264621065No Hit
GCTGCGTTCTTCATCGATGCTACCCTTATCTGCAGCAGCTGGAATCTCTG2480.37840064694304154No Hit
GCTGCGTTCTTCATCGATGCCAGCGCTGCGGGGTCGGGGATATCGGCGAG2410.3677199835212622No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA2310.35246189291872015No Hit
GCTGCGTTCTTCATCGATGCGTACATAAACTATGTAGGAACGCCCGCGCT2120.32347152077389035No Hit
GCTGCGTTCTTCATCGATGCTTCTGTTACGCAGGCTGCGTTCTTCATCGA1490.22734554997787576No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT1440.21971650467660478No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTT1310.19988098689330017No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT1270.19377775065228336No Hit
GCTGCGTTCTTCATCGTTTCGAAACGCCTAGGCATCCGCCGTATGCCCTT1210.18462289629075818No Hit
GCTGCGTTCTTCATCGATGCTTCTGTTACGCACGCTGCGTTCTTCATCGA1080.16478737850745356No Hit
GCTGCGTTCTTCATCGATGTCTTCCTTTGTAGCATCAGCCGGGAAGGTCA1040.1586841422664368No Hit
GCTGCGTTCTTCATCGATGCCAAGGAGCGGATTAAACCCTTCAGTAAGAC1030.15715833320618258No Hit
GCTGCGTTCTTCATCGATGCACTGATCTCGCGGATGAGCGAGCAGCCCAT950.14495186072414898No Hit
GCTGCGTTCTTCATCGATGCACACAGGAGCTCAGAATATACCTAGTGAAG910.13884862448313218No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT880.13427119730236958No Hit
GCTGCGTTCTTCATCGATACGAGAACCAAGAGATCCATTGTTAAAAGTTT870.13274538824211538No Hit
GCTGCGTTCTTCATCGATGCTGTCCGATGTTACGGTAGTGGGCGTCTTCA850.129693770121607No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT850.129693770121607No Hit
TCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT830.12664215200109857No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT830.12664215200109857No Hit
GCTGCGTTCTTCATCGATGCCACCACCTCAATGCCATCTCGCTGATCTGG810.12359053388059017No Hit
GCTGCGTTCTTCATCGATGCGCTGTCGGTTGGCTTTACCATTGCCAACTA690.10528082515753978No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTAAAAGTTG680.10375501609728559No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)