FastQCFastQC Report
Sun 21 May 2023
EGAF00007832621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76487
Sequences flagged as poor quality0
Sequence length247-250
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC4192854.81715847137421No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA1899124.82905591799914No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC19542.5546824950645206No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC15051.9676546341208312No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC12031.5728162955796412No Hit
GGAAGTAAAAGTCGTAACAAGGCGGATAAGAAAAACTTTGACGATTTACA5920.7739877364780944No Hit
GGAAGTAAAAGTCGTAACAAGGCGTAAAATCTCAGGGATGTTGCGCCCAT5800.7582987958738087No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC4730.6184057421522612No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGTTGAAAAAGCTGGTAATTGTCAT4500.5883352726607136No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC4060.530809157111666No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACGGGAACGTGCGGCTGGATC3610.4719756298455947No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA3470.4536718658072613No Hit
GGAAGTAAAAGTCGTAACAAGGCTACGGAAAAGAAAGTTTTGGTGGGCAA3300.43144586661785667No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC3170.4144495142965471No Hit
GGAAGTAAAAGTCGTAACAAGGGTGAGCAGAAAGGCTTTGAAAGAGTAAG2440.31900845895380914No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA2080.27194163714095204No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC1820.23794893249833304No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC1750.2287970504791664No Hit
GGAAGTAAAAGTCGTAACAAGGCTGAGTTGCACAAACTCTTTTTTGATTT1630.21310810987488069No Hit
GGAAGTAAAAGTCGTAACAAGGGGCGTAAGTTCAAAGTCAATATCGTCTA1620.21180069815785688No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT1540.20134140442166645No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACAACTCTCCGGCA1420.1856524638173807No Hit
GGAAGTAAAAGTCGTAACAAGGGCACAGCATATCGGGGATTAGTCTATTC980.1281263482683332No Hit
GGAAGTAAAAGTCGTAACAAGGCAATATGCGCCAAACCGCCCCTGCACAG980.1281263482683332No Hit
GGAAGTAAAAGTCGTAACAAGGGACTAAAGACGCATGGGAGGCAGGCAAG970.12681893655130938No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG890.1163596428151189No Hit
GGAAGTAAAAGTCGTAACAAGGGATGGAGATTAGTCAATATTATCGAAAG880.1150522310980951No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGAAACCTCTCCGGCA850.11112999594702368No Hit
GGAAGTAAAAGTCGTAACAAGGATTTTGACCTGGGGACCGTCCTGGATAC840.10982258422999987No Hit
GGAAGTAAAAGTCGTAACAAGGACAGAGTAGTTGAGGTGGTACTTCTGCT830.10851517251297606No Hit
GGAAGTAAAAGTCGTAACAACAGCAACGGTTTCAGTCCATATAGGACGCA800.10459293736190464No Hit
GGAAGTAAAAGTCGTAACAAGGCTAAGAAGGACTACAGAAAGAAACAGGG800.10459293736190464No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)