FastQCFastQC Report
Sun 21 May 2023
EGAF00007832627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175463
Sequences flagged as poor quality0
Sequence length247-250
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC11623566.2447353573118No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA2722015.513242108022773No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC50662.88721838792224No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG40852.3281261576514707No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG30111.7160313000461633No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACTTGCGGAAGGATC11400.6497096253911081No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC8570.4884220604913857No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT6610.37671759858203724No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAACCAACATGACTGATGAAGAAAAA5610.31972552617930844No Hit
GGAAGTAAAAGTCGTAACAAGGTACCCGAATGCCGGATTCCTCGCTAACA5590.31858568473125387No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC4520.2576041672603341No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC3290.1875039182049777No Hit
GGAAGTAAAAGTCGTAACAAGGGCGGGTGTGGTGTTCCAAGCAAGGCAGG3160.18009494879262294No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC3140.17895510734456838No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC3120.1778152658965138No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGTCAGCCAATCGACGCAATCG2700.1538785954873677No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGAAGGTTTGGTACACGTTTCCGAA2610.14874930897112212No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG2460.14020049811071278No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAAAGGACCGGAATCTGCTTCATACG2290.13051184580224892No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACGGGAACGTGCGGCTGGATC2260.12880208363016704No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC2200.1253825592860033No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC2150.12253295566586689No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGAAAGGATC2100.11968335204573044No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC2080.11854351059767587No Hit
GGAAGTAAAAGTCGTAACAAGGTGTACATAAAAGGCGACGGCGGAAAATG2060.1174036691496213No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC1950.11113454118532112No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC1900.10828493756518467No Hit
GGAAGTAAAAGTCGTAACAAGGAATTGACTGGAGGAAGATGGATAATAGA1860.10600525466907552No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC1790.10201580960088452No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)