FastQCFastQC Report
Sun 21 May 2023
EGAF00007832631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71869
Sequences flagged as poor quality0
Sequence length247-250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC2593636.08788211885514No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA1963527.32054154085906No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTTAAGGTTTCGGATAAAGCA801911.15780099903992No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC52837.350874507784997No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT18612.589433552713966No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG7761.0797423089231797No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA5610.7805869011674018No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC4300.5983108155115557No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC4080.5676995644853832No Hit
GGAAGTAAAAGTCGTAACAAGGAATTGTTGAAAAAGCTGGTAATTGTCAT3500.4869971754163826No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG2640.36733501231407145No Hit
GGAAGTAAAAGTCGTAACAAGGTAGTGATGGACAAACCGGCGAAAAAGAC2290.3186352947724332No Hit
GGAAGTAAAAGTCGTAACAAGGATAGAGACTTGGTATGAGTGCTGGAAGG2210.3075039307629159No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC2160.30054682825696755No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT1920.26715273622841557No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC1900.26436989522603627No Hit
GGAAGTAAAAGTCGTAACAAGGACGTGCTCGTGCTGTGCCCTAAGAAGCT1880.2615870542236569No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGAA1810.2518471107153293No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTATCCGGCA1810.2518471107153293No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC1680.23375864419986364No Hit
GGAAGTAAAAGTCGTAACAAGGTACCCGAATGCCGGATTCCTCGCTAACA1670.232367223698674No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC1650.22958438269629466No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC1630.22680154169391534No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTTAAGGTTTCGGATAAAGTA1580.219844439187967No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGTAAGGATC1450.20175597267250134No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAAACTGCGGAAGGATC1390.19340744966536336No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTTCGGAAGGATC1360.18923318816179438No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC1350.1878417676606047No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC1170.16279619863919073No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCAGTAGGTGAACATGCGGAAGGATC1140.15862193713562175No Hit
GGAAGTAAAAGTCGTAACAAGGACAAGCAATCGGGATTTTAGCCGGTGAA1110.15444767563205275No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC940.13079352711182846No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC860.11966216310231115No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA770.10713937859160418No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA750.10435653758922483No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACATCTCCGGCA720.10018227608565584No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)