FastQCFastQC Report
Sun 21 May 2023
EGAF00007832633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107306
Sequences flagged as poor quality0
Sequence length247-250
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC6136957.19065103535683No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC49774.6381376623860735No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC41683.8842189625929584No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA26772.494734683987848No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG26182.439751738020241No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGAAACAGAATCCGAAAAGAGCACA14741.3736417348517325No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC14351.337297075652806No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA13931.2981566734385777No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC12761.1891226958417982No Hit
GGAAGTAAAAGTCGTAACAAGCCACAGAAGAAATGAACGATCGTAGGGAT11831.1024546623674352No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCGTCGTTACC11651.085680204275623No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA11601.0810206325834528No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCGAATCTAATAATGAAGAATCAG11321.0549270311073005No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC10190.9496207108642574No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG6220.579650718505955No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC5640.5255996868767823No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC5600.5218720295230462No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT5060.47154865524760964No Hit
GGAAGTAAAAGTCGTAACAAGAAATGATAATTTAAACAAAAATACAGCTA4770.44452313943302335No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT4660.4342720817102492No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA4510.42029336663373906No Hit
GGAAGTAAAAGTCGTAACAACTTCCAGGTTTCAGGTTGGAAAGTCCCTCA4440.4137699662647009No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGACAAGCTGATGATTGTGGGAGCAT4430.41283805192626694No Hit
GGAAGTAAAAGTCGTAACAAGGCTAAATCTATTATGGCAGGTGGAGGAGT4040.3764933927273405No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC3740.3485359625743202No Hit
GGAAGTAAAAGTCGTAACAAGGTAATCGTAGGTGAACCTGCGTTTGGATC2910.27118707248429724No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA2900.2702551581458632No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC2610.2432296423312769No Hit
GGAAGTAAAAGTCGTAACAAGGACAAGCAATCGGGATTTTAGCCGGTGAA2570.23950198497754085No Hit
GGAAGTAAAAGTCGTAACAAGGCGGTCATGGACAAGAAGCAGGTCGCTGT2450.22831901291633272No Hit
GGAAGTAAAAGTCGTAACAAGGATAAAACCCAAAATAAGCCGGCAGTTGT2360.21993178387042664No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGTCAGCCAATCGCCGCAATCG2210.20595306879391648No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC2180.20315732577861445No Hit
GGAAGTAAAAGTCGTAACAAGGCCCACCAAATCGGGATATCGGCACTTAC2100.19570201107114235No Hit
GGAAGTAAAAGTCGTAACAAGGGTCGCACGGTCACGGATCTCACCCACGA2070.1929062680558403No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG1800.16774458091812203No Hit
GGAAGTAAAAGTCGTAACAAGGCGTAGAGGAATTAGCGCAGAATCAGAAA1700.1584254375337819No Hit
GGAAGTAAAAGTCGTAACAAGGCTAAGAAGGACTACAGAAAGAAACAGGG1690.15749352319534787No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC1610.15003820848787577No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGTTGAACCTGCGGAAGGATC1550.14444672245727172No Hit
GGAAGTAAAAGTCGTAACAAGGTTGGCGGGATTTACACAGTTCTTTCGAC1450.1351275790729316No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC1430.1332637503960636No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC1400.13046800738076156No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGTATC1350.12580843568859149No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAAACTGCGGAAGGATC1270.1183531209811194No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC1260.1174212066426854No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATA1240.11555737796581737No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACTGAATCCGAAAAGAGCACA1210.11276163495051535No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAATGATC1200.11182972061208134No Hit
GGAAGTAAAAGTCGTAACAAGGGCAGAAGGAATTCAGAAGAGCCAGGGTG1150.10717014891991128No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)