FastQCFastQC Report
Sun 21 May 2023
EGAF00007832640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99055
Sequences flagged as poor quality0
Sequence length235-238
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT4983550.31043359749634No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT2127921.48200494674676No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG79468.021806067336328No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG40244.062389581545606No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA9240.9328151027207108No Hit
GCTGCGTTCTTCATCGATGTGTTTTGGCAATGGCAACTGGACTAAGATTC7670.7743172984705466No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT6920.6986017868860734No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG5940.5996668517490283No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT5590.5643329463429407No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTT4900.4946746756852254No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG3810.3846347988491242No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT3580.36141537529655243No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG3080.31093836757357024No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT3030.305890666801272No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT2720.27459492201302305No Hit
GCTGCGTTCTTCATCGATACGAGAACCAAGAGATCCATTGTTAAAAGTTT2570.2594518196961284No Hit
TCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT2510.25339457876937055No Hit
GCTGCGTTCTTCATCGATGCATGGTCATCATGTGGTGAGAAATCTTCTAA2400.24228963707031448No Hit
GCTGCGTTCTTCATCGATGCCGCCTCCAGACGGAATCTGGAACTCACAGA2020.20392711120084803No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT2010.20291757104638838No Hit
GCTGCGTTCTTCATCGATGTGTTTTGGTAAGGGTAACTGCACCAAAATAC1960.19786987027409017No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG1920.19383170965625157No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT1740.175659986875978No Hit
GCTGCGTTCTTCATCGATGGGGGCGGCGACCACGTCCTTATTTCTAGGGA1600.16152642471354298No Hit
GCTGCGTTCTTCATCGATGCTCATCAGGGATATTGGCCTAAAATTTTCTT1550.15647872394124476No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT1370.1383070011609712No Hit
GCTGCGTTCTTCATCGATGCACACAGGAGCTCAGAATATACCTAGTGAAG1310.1322497602342133No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTTCCGTCTTTTCTTCTTGTTT1230.12417343899853617No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGATGCCGGACGATCGGGCAAACT1200.12114481853515724No Hit
GCTGCGTTCTTCATCGATGCCCACGCCGGTACGCTCGGCACGCTCGGCGC1160.11710665791731865No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG1150.11609711776285903No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT1070.10802079652718188No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAGC1060.10701125637272223No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT1020.10297309575488364No Hit
GCTGCGTTCTTCATCGATGCCTAGTGCCAAGGCATCCACCGTATGCCCTT1020.10297309575488364No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)