FastQCFastQC Report
Sun 21 May 2023
EGAF00007832641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84830
Sequences flagged as poor quality0
Sequence length247-250
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC3407940.173287751974534No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA1224114.430036543675588No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG26923.17340563479901No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA20122.371802428386184No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA16311.922668867146057No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT16241.9144170694329836No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG14631.7246257220323No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT12331.4534952257456089No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC11781.388659672285748No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC11391.342685370741483No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGTTGAAAAAGCTGGTAATTGTCAT11301.3320759165389602No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC9511.1210656607332312No Hit
GGAAGTAAAAGTCGTAACAAGGAAGACAACTGAGGAATGGTGATGTTGCG8681.0232229164210773No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC8400.9902157255687846No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA7730.9112342331722268No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC7590.8947306377460804No Hit
GGAAGTAAAAGTCGTAACAAGGTTGATAGTAGTAAATGAGTTGTTGCCAC6090.7179064010373689No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC5520.6507131910880585No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG5330.6283154544382883No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCGAT5330.6283154544382883No Hit
GGAAGTAAAAGTCGTAACAAGGCAAAAATAGACAAGTGGGACTATATCAA5030.592950607096546No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG4230.4986443475185665No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGATAGTCCTGAAGTATCTCTTCA4180.4927502062949428No Hit
GGAAGTAAAAGTCGTAACAAGGTTCCAGACCGAAGAGATAGAGCTGCGAT4150.4892137215607686No Hit
GGAAGTAAAAGTCGTAACAAGAGATGGAGATTAGTCAATATTATCGAAAG3880.4573853589532005No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC3480.41023222916421076No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC3170.3736885535777437No Hit
GGAAGTAAAAGTCGTAACAAGCGGTAAGAAGAATACGATTAGCACTTGAC3160.37250972533301896No Hit
GGAAGTAAAAGTCGTAACAAGGTTGGCGGGATTTACACAGTTCTTTCGAC2660.3135683130967818No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC2650.31238948485205703No Hit
GGAAGTAAAAGTCGTAACAAGGTGTACATAAAAAGCGACGGCGGAAAATG2580.30413768713898387No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC2580.30413768713898387No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT2560.30178003064953435No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC2400.28291877873393845No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC2240.26405752681834255No Hit
GGAAGTAAAAGTCGTAACAAGGCTAAATCTATTATGGCAGGTGGAGGAGT2150.25344807261581986No Hit
GGAAGTAAAAGTCGTAACAAGGCCCACCAAATCGGGATATCGGCACTTAC2100.24755393139219614No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC2080.24519627490274665No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG2040.24048096192384769No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGTAAAGGAATAAAAAACAACCGCG1770.2086525993162796No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGAATACGACGAGAGGCGACATCCT1720.20275845809265589No Hit
GGAAGTAAAAGTCGTAACAAGGCTTTGGAGTAGACCATTTAGTGAATGGA1630.1921490038901332No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC1540.18153954968761052No Hit
GGAAGTAAAAGTCGTAACAAGGCTAAGAAGGACTACAGAAAGAAACAGGG1510.1780030649534363No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC1460.17210892372981257No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC1410.16621478250618885No Hit
GGAAGTAAAAGTCGTAACAAGGGCCGACAAGTTAGCGGTTCTGAATGCTC1380.16267829777201462No Hit
GGAAGTAAAAGTCGTAACAAGGAACGTTCAGCGATAGCTGCAGGTTTCCA1370.16149946952728989No Hit
GGAAGTAAAAGTCGTAACAAGGATTTGCGCAGGTTGAAGGTGGTGTGCAC1360.16032064128256512No Hit
GGAAGTAAAAGTCGTAACAAGGGAAGGTTGTCATGCTACAGAGTGGTCCT1300.15324767181421667No Hit
GGAAGTAAAAGTCGTAACAAGGAAATGCGGATCCTCATGTTGGGCCTGGA1280.15089001532476717No Hit
GGAAGTAAAAGTCGTAACAAGGGCAGAAGGAATTCAGAAGAGCCAGGGTG1240.1461747023458682No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACAACTCTCCGGCA1170.137922904632795No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC1110.13084993516444654No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGCAGAAAAGCATGATGATAGCCC1090.12849227867499705No Hit
GGAAGTAAAAGTCGTAACAAGGCAGCAGTCTTGCCCTGAACATGGTTTCC1010.1190616527171991No Hit
GGAAGTAAAAGTCGTAACAAGGGTGGCAAGTGAGGGAGGATGAGAAGTGC1000.11788282447247435No Hit
GGAAGTAAAAGTCGTAACAAGGACAGAGCAACGTATCAGGCGGCGGTGGC990.11670399622774962No Hit
GGAAGTAAAAGTCGTAACAAGGAAATAATTCGACGCCGCCGGGGCGATGT930.10963102675940115No Hit
GGAAGTAAAAGTCGTAACAAGGGTTAGTAGATGTTTTAAAATTTTCATTT930.10963102675940115No Hit
GGAAGTAAAAGTCGTAACAAGGAGGCCAAGAAGATCGTCGTGCAGTCCAT920.1084521985146764No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGCTACCAAAGCTCCTCCCTCTTCA870.10255805729105269No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC860.10137922904632794No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTTCGGAAGGATC850.1002004008016032No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)