FastQCFastQC Report
Sun 21 May 2023
EGAF00007832642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84830
Sequences flagged as poor quality0
Sequence length235-238
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT1986423.416244253212305No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1182113.934928680891195No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT1080812.74077566898503No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG26743.1521867263939645No Hit
GCTGCGTTCTTCATCGATGCTCATCAGGGATATTGGCCTAAAATTTTCTT21102.487327596369209No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT14111.6633266533066133No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAAC13081.5419073440999647No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT12041.4193092066485913No Hit
GCTGCGTTCTTCATCGATGCTCCATGTTATATGGATTTCATAACTAATAC11621.369798420370152No Hit
GCTGCGTTCTTCATCGGCGCAGTCCTTGTCATTTACTACAAACAAATTTC10701.2613462218554756No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCTCCTGTCCGAGGT10331.2177295768006602No Hit
GCTGCGTTCTTCATCGATGTCTTCCTTTGTAGCATCAGCTGGGAAAGTCA9901.167039962277496No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG9681.1411057408935519No Hit
GCTGCGTTCTTCATCGATGCAACGCATGAGGACGCTGCGTTCTTCATCGA7000.8251797713073205No Hit
GCTGCGTTCTTCATCGATGCACACAGGAGCTCAGAATATACCTAGTGAAG6860.808676175881174No Hit
GCTGCGTTCTTCATCGATGCACCTTTCAATCTTAAATACTACAAAAAGGG6120.7214428857715431No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT6110.7202640575268183No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT5800.6837203819403513No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT5470.6448190498644347No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT4610.5434398208181068No Hit
GCTGCGTTCTTCATCGATGCCTTCCTGAACGTGGACAAAGGATTGGTCAA4310.5080749734763644No Hit
GCTGCGTTCTTCATCGATGCCTTCCTTAACGTGGACAAAGGATTGGTCAA4290.505717316986915No Hit
GCTGCGTTCTTCATCGATGCCACCACCTCAATGCCATCTCGCTGATCTGG4180.4927502062949428No Hit
GCTGCGTTCTTCATCGATGCTAGGCTTTTTACTTCTTCTTTTGCTTCAGC4160.4903925498054933No Hit
GCTGCGTTCTTCATCGATGCTTCTTGAATAGTAACCTTTTCTAAGCACTA4120.48567723682659436No Hit
GCTGCGTTCTTCATCGATGCAACGCATGAGGAGGCTGCGTTCTTCATCGA4100.48331958033714484No Hit
GCTGCGTTCTTCATCGATGCGGATGTAGGAGCCGTCGGGACGACGAACTT3840.4526700459743015No Hit
GCTGCGTTCTTCATCGATGCCCGCATGCCTGTTCGTCGCTACGAGGTGCT3840.4526700459743015No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT3780.44559707650595304No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA3670.4326299658139809No Hit
GCTGCGTTCTTCATCGATGCACGCATGAGGACGCTGCGTTCTTCATCGAT3440.40551691618531177No Hit
GCTGCGTTCTTCATCGATGCGGATACGCTGAGCACCGTGACGCCGGACAA3380.3984439467169633No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG3320.39137097724861486No Hit
GCTGCGTTCTTCATCGATGCAACGCATGAGGATGCTGCGTTCTTCATCGA3240.38194035129081694No Hit
GCTGCGTTCTTCATCGATGCTCATTATCTTTTTTTTTTGGTAATAGTTAT3190.37604621006719324No Hit
GCTGCGTTCTTCATCGACGACAGTCTCGACTATCTCGAGCGAATCGACGA3050.3595426146410468No Hit
GCTGCGTTCTTCATCGAAACGGATATAACTACCATCTTTTCTTCTGGTCT2770.326535423788754No Hit
GCTGCGTTCTTCATCGATGCAGGTGAAGAGGTCTATGGCGGACAGGTCGT2760.32535659554402924No Hit
GCTGCGTTCTTCATCGATGCCTCATCATAGTCGCCATAACCATGAAGGCT2600.30649534362843334No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG2540.2994223741600849No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC2450.2888129199575622No Hit
GCTGCGTTCTTCATCGATGCACGCATGAGGAGGCTGCGTTCTTCATCGAT2340.27584580926559No Hit
GCTGCGTTCTTCATCGATTTCACCCAACAATGCTTCAGGAGAAACGACAA2300.271130496286691No Hit
GCTGCGTTCTTCATCGATGCCTTTGCCGAAGACGAACCGTGGCGGGCCGT2220.2616998703288931No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAGC2190.25816338559471885No Hit
GCTGCGTTCTTCATCGATGCCATTGAAAGTATACCAATAGCTTTCTCTAT2110.2487327596369209No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTTCCGTCTTTTCTTCTTGTTT2090.2463751031474714No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT1910.225156194742426No Hit
GCTGCGTTCTTCATCGATGCATATGTGGTGTGGCCCACATTTCTGTATGC1890.22279853825297652No Hit
GCTGCGTTCTTCATCGATGCTTGCAAGAAATCGCGTCTTAAACAGTTTGA1780.20983142756100434No Hit
GCTGCGTTCTTCATCGATGCAACGCATGAGGAAGCTGCGTTCTTCATCGA1740.20511611458210538No Hit
GCTGCGTTCTTCATCGATGCGCCGTCATTGACGAATTGCACTTCGGCAGT1680.1980431451137569No Hit
GCTGCGTTCTTCATCGATGCACGCATGAGGATGCTGCGTTCTTCATCGAT1490.1756454084639868No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA1460.17210892372981257No Hit
GCTGCGTTCTTCATCGATGCTCTTGCCGAACGCTACGGCGTCTCGTACTG1340.15796298479311563No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCATTGCCGAGAGTCG1340.15796298479311563No Hit
GCTGCGTTCTTCATCGATGCCAATACGGTAGCGACGACGCTGCACATTTG1310.1544265000589414No Hit
GCTGCGTTCTTCATCGATGCTGAGTAAAGCAGACTGGCCACCTGTCTTCT1300.15324767181421667No Hit
GCTGCGTTCTTCATCGATGCGAGCAAGCGACGTTATGTGGTTTATATTTG1270.14971118708004244No Hit
GCTGCGTTCTTCATCGTTTCGAAACGCCTAGGCATCCGCCGTATGCCCTT1260.14853235883531768No Hit
GCTGCGTTCTTCATCGATGCGGTCGCGGTCGGCGCAGTCCACTACGAAGA1260.14853235883531768No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCTGTTGCCGAGAGTCG1190.1402805611222445No Hit
GCTGCGTTCTTCATCGATGCGTCGATGGTAGGTCATCTGGGTGCGGAAGC1190.1402805611222445No Hit
GCTGCGTTCTTCATCGATGCAACGCATGAGGACGCTGCGTTCTTCATCGG1180.13910173287751973No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT1160.13674407638807026No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTT1110.13084993516444654No Hit
GCTGCGTTCTTCATCGATGCAACGCATGAGGAGGCTGCGTTCTTCATCGG1070.12613462218554755No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCCGACGATCGGGCAAACT1050.12377696569609807No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTAG1020.12024048096192384No Hit
GCTGCGTTCTTCATCGATGCTCATTATCTTTTTTTTTTTTGGTAATAGTT990.11670399622774962No Hit
GCTGCGTTCTTCATCGATGCCAAGGCAAATGAAAATGAGGTGCAAACAGG980.11552516798302487No Hit
GCTGCGTTCTTCATCGATGCAGACAACGATCAAATCCAAGCCGCCCATGA970.11434633973830012No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAAAACAGGAACAACACAATGTGTT960.11316751149357537No Hit
GCTGCGTTCTTCATCGATGCACTGGGGAGCCCCTGCTTCAAACCTGCAGG960.11316751149357537No Hit
GCTGCGTTCTTCATCGATGCACGCATGAGGAAGCTGCGTTCTTCATCGAT940.11080985500412589No Hit
GCTGCGTTCTTCATCGATGCTCGTGCGCCGTTCCGCTACGATGGGTCTCA930.10963102675940115No Hit
GCTGCGTTCTTCATCGATGCGTGCAGGGTGAATGCGACCATCCTGTACCA910.10727337026995167No Hit
GCTGCGTTCTTCATCGATGTGTTTTGGTAATGGTAACTGCACCAAAATCC870.10255805729105269No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAAAGTCG860.10137922904632794No Hit
GCTGCGTTCTTCATCGATGCTGGGGCTGGCATTGATAGATTTGGCTACAG850.1002004008016032No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)