FastQCFastQC Report
Sun 21 May 2023
EGAF00007832645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73247
Sequences flagged as poor quality0
Sequence length247-250
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC1612322.0118230098161No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC1455319.868390514287274No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC1112715.191065845700166No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT739610.09734187065682No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA59898.176444086447226No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC17922.4465165808838587No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC15432.1065709175802425No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC10111.3802613076303467No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC6340.8655644599778831No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC4520.6170901197318662No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA4510.6157248761041407No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC4280.5843242726664574No Hit
GGAAGTAAAAGTCGTAACAAGGATAAAACCCAAAATAAGCCGGCAGTTGT3980.5433669638346963No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGGGAACGTGGGGATGGATC3430.4682785643098011No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC3420.46691332068207575No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG3060.41776455008396246No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC2530.345406637814518No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC2480.3385804196758912No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGGCATGAAACTCACCGAAAGCTAC2420.330388957909539No Hit
GGAAGTAAAAGTCGTAACAAGGATGGCATCATTGGTTACGTTCTTGCAAT2410.3290237142818136No Hit
GGAAGTAAAAGTCGTAACAAGTTATGTAGATGAAGAACGCAGCAGCTAAG1880.2566658020123691No Hit
GGAAGTAAAAGTCGTAACAAGGCTGAGTTGCACAAACTCTTTTTTGATTT1680.22936092945786174No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCAGCTAAGCC1530.20888227504198123No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGTGAACGTGCGGATGGACT1430.19522983876472758No Hit
GGAAGTAAAAGTCGTAACAAGGACGGCATTATTGGTTACGTTCTTGCAAT1300.17748167160429779No Hit
GGAAGTAAAAGTCGTAACAAGGCGTAGAGGAATTAGCGCAGAATCAGAAA1180.16109874807159338No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGTTGAAAAAGCTGGTAATTGTCAT1080.1474463117943397No Hit
GGAAGTAAAAGTCGTAACAAGGCGGCAGCCGCAGTGGAATCCGTCGTGCG1070.14608106816661434No Hit
GGAAGTAAAAGTCGTAACAAGGTTAAGAGAGGGCCGTTTTCCTTATTAGG1000.13652436277253674No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA910.12423717012300846No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG870.11877619561210698No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC870.11877619561210698No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGTGGCATAGATGGTGATAACTTGTA820.11194997747348014No Hit
GGAAGTAAAAGTCGTAACAAGGACGAGGTGGACATCCGCGTGGCGATCAG810.11058473384575476No Hit
GGAAGTAAAAGTCGTAACAAGAGGAGTGTAGACGTCCCAGAAGTGCAGCT800.10921949021802942No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)