FastQCFastQC Report
Sun 21 May 2023
EGAF00007832650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87291
Sequences flagged as poor quality0
Sequence length235-238
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT3417039.14492903048424No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT1032711.83054381322244No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAAC49505.670687699762862No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG27303.127470185929821No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG25632.9361560756549934No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACTAATGTGT10651.22005705055504No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT9881.1318463530031733No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTGAAAGTTT9621.1020609226609845No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT8700.9966663229886242No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAGC8630.9886471686657273No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAAAACAGGAACAACACACACGTGT6740.7721300019475089No Hit
GCTGCGTTCTTCATCGATGCCTTCCTTAACGTGGACAAAGGATTGGTCAA4890.5601952091280887No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCA4770.546448087431694No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT4740.5430113070075954No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT4740.5430113070075954No Hit
GCTGCGTTCTTCATCGATGCCTATCACACCATTTAAGATGTTTTCCTGTG4740.5430113070075954No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTT4630.5304097787859No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGCTTTTTTCTTTGCGAT4570.5235362179377027No Hit
GCTGCGTTCTTCATCGATGCACTCAACAGTTGGCAACTTGTCAAGGAACT4480.5132258766654065No Hit
GCTGCGTTCTTCATCGATGCGAGAACCACGAGATCCGTTGTTGAAAGTTT4460.5109346897160073No Hit
GCTGCGTTCTTCATCGATGCTGGACCACCCCAGCTAATAATCGCATGTCT4030.46167417030392593No Hit
GCTGCGTTCTTCATCGATGCCTTTGCCGAAGACGAACCGTGGCGGGCCGT3780.4330343334364367No Hit
GCTGCGTTCTTCATCGATGCGTACATAAACTATGTAGGAACGCCCGCGCT3620.4147048378412436No Hit
GCTGCGTTCTTCATCGATGCACACAGGAGCTCAGAATATACCTAGTGAAG3600.4124136508918445No Hit
GCTGCGTTCTTCATCGATGCTCGCGTCCACTATGCAGTTCACAACCAACC3510.40210330961954843No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG3370.38606500097375446No Hit
GCTGCGTTCTTCATCGATGCCGTATTGGGCGTAGCGATGGCGTGGCTGCT3080.352842790207467No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA3060.35055160325806783No Hit
GCTGCGTTCTTCATCGATGCACGATCCTTCCTCGCTGCAGGATCCTTCCT2950.3379500750363726No Hit
GCTGCGTTCTTCATCGATGCTGGAACCAAGAGATCCGTTGTTGAAAGTTT2900.33222210766287474No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT2700.3093102381688834No Hit
GCTGCGTTCTTCATCGATGCGCCTGACAAGGATGCCATTGAGAAGAATAT2690.3081646446941838No Hit
GCTGCGTTCTTCATCGATACGAGAACCAAGAGATCCATTGTTAAAAGTTT2650.3035822707953855No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT2630.3012910838459864No Hit
GCTGCGTTCTTCATCGATGCCTCCGCCCATAGCCTGAAAATATCCCAGCT2330.2669232796049994No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCATTGCCGAGAGTCG2320.2657776861302998No Hit
GCTGCGTTCTTCATCGATGCACTGATCTCGGAGATGAGCGAGCAGCCTAT2290.2623409057062011No Hit
GCTGCGTTCTTCATCGATGGCTTGTTTTGCAGCTGGCTTCGCTTGCGCTG2220.2543217513833041No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG2170.24859378400980628No Hit
GCTGCGTTCTTCATCGATGCGGCATACAAGGCCTGATGCCATGGGAAGAC2150.24630259706040714No Hit
TCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT2110.24172022316160885No Hit
GCTGCGTTCTTCATCGATGCCCATACGAATGATCGGAAGGTGGAAAAGGG2040.23370106883871192No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT1940.2222451340917162No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCTAGATATCCGTTGCCGAGAGTCA1920.21995394714231709No Hit
GCTGCGTTCTTCATCGAAACGGATATAACTACCATCTTTTCTTCTGGTCT1830.20964360587002098No Hit
GCTGCGTTCTTCATCGATATGTTTGGGTAAAGGTAACTGAACCAAAATCC1750.20047885807242444No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACATGCGTGT1730.19818767112302527No Hit
GCTGCGTTCTTCATCGATGCGGAACTTCGCGGACTCAATTCGCGCCTGCA1730.19818767112302527No Hit
GCTGCGTTCTTCATCGATGCCGCCGATTCCGGAGAAGGCCTGCTCGGGGA1610.18444054942663046No Hit
GCTGCGTTCTTCATCGATGCCGCGCGCCGCCACGTCGGTGGCCACGAGGA1590.18214936247723132No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT1530.17527580162903392No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATACGTTGTTGAAAGTTT1440.16496546035673781No Hit
GCTGCGTTCTTCATCGATGCCAAGGATAATTTTAGACGCCCCACAGAGCG1400.16038308645793953No Hit
GCTGCGTTCTTCATCGGCGCTTACTACCTAGGCATCCTTTGTGTGCCCTT1360.15580071255914127No Hit
GCTGCGTTCTTCATCGATTTCGCCCAACAATGCTTCAGGAGAAACGACAA1320.15121833866034298No Hit
GCTGCGTTCTTCATCGCAGCGACAGCCGTGAGCGCGGCGCCGTAGACATT1320.15121833866034298No Hit
GCTGCGTTCTTCATCGATGCTGTCTCAAACAGCAAAGTGGAGAATAAGGA1280.14663596476154472No Hit
GCTGCGTTCTTCATCGATCGTCTTCCTTATTCTCAGCCAAAATAAACTTC1270.14549037128684517No Hit
GCTGCGTTCTTCATCGATGCGAGGTTGTTGAATGCGCGAGCGTCAGAAGG1240.14205359086274644No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA1220.1397624039133473No Hit
GCTGCGTTCTTCATCGATCACGGCATGGGCATCGTCATTGGTGAGACCGC1210.13861681043864774No Hit
GCTGCGTTCTTCATCGATGCCGTTCAGGTAGCAGGAAAACTTCCTATTCA1200.13747121696394818No Hit
GCTGCGTTCTTCATCGATGTGTTTTGGCAATGGAAGCTGAACCAAAATCC1190.13632562348924862No Hit
GCTGCGTTCTTCATCGATGCGTCTGAGAATTTCTCGGCTTCTACGGGAGC1180.13518003001454904No Hit
GCTGCGTTCTTCATCGATGGCTTGTTTTGCCGCTGGCTTCGCTTGGGCTG1130.1294520626410512No Hit
GCTGCGTTCTTCATCGATGCTGGAGCCAAGAGATCCGTTGTTGAAAGTTT1060.12143290831815423No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT1060.12143290831815423No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG1060.12143290831815423No Hit
GCTGCGTTCTTCATCGATGCATATGTGGTGTGGCCCACATTTCTGTATGC980.11226816052055767No Hit
GCTGCGTTCTTCATCGATGCATGATGATTCCTTGCTGCACGATCCTTCCT970.1111225670458581No Hit
GCTGCGTTCTTCATCGATGCGGCCGCCGTTCCCGCCGCGTCGTGTGTGGC970.1111225670458581No Hit
GCTGCGTTCTTCATCGCTGTCAGATACCTTGGCATCCTTCGTATGCCCTT930.10654019314705983No Hit
GCTGCGTTCTTCATCGATGCACCTGAGCCAATTTTTGGCTTGGCGGTAAC910.10424900619766071No Hit
GCTGCGTTCTTCATCGATGCGGTTGGCGTCGTCGATGGTATCGTTATACT910.10424900619766071No Hit
GCTGCGTTCTTCATCGATGCTCCATGTTATATGGATTTCATAACTAATAC910.10424900619766071No Hit
GCTGCGTTCTTCATCGATGCGAGAACCTAGAGATCCGTTGTTGAAAGTTT900.10310341272296113No Hit
GCTGCGTTCTTCATCGATGCCTGTGAACCGATAACCGCGGTAGTAATAGA900.10310341272296113No Hit
GCTGCGTTCTTCATCGATGCCCTGGTCCCCGACGCACGCGCCTTTCGTCT890.10195781924826157No Hit
GCTGCGTTCTTCATCGTTTCGAAACGCCTAGGCATCCGCCGTATGCCCTT880.100812225773562No Hit
GCTGCGTTCTTCATCGATGCTAGGCTTTTTACTTCTTCTTTTGCTTCAGC880.100812225773562No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)