FastQCFastQC Report
Sun 21 May 2023
EGAF00007832662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70491
Sequences flagged as poor quality0
Sequence length235-238
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT2506035.55063767005717No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG2149130.487579974748552No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT32144.559447305329758No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCT26943.8217644805719884No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT20472.9039168120753005No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG14722.088209842391227No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT12871.825764991275482No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG11281.6002042813976252No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT7861.115035962037707No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT5740.8142883488672312No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC5480.7774042076293427No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT5300.7518690329261892No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT5290.7504504121093474No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG5040.7149848916883006No Hit
GCTGCGTTCTTCATCGATGCCAAGGAGCGGATTAAACCCTTCAGTAAGAC3770.5348200479493836No Hit
GCTGCGTTCTTCATCGATGCTGGAACCAAGAGATCCGTTGTTGAAAGTTT3220.456795903023081No Hit
GCTGCGTTCTTCATCGAAGAAAGGCACTGGTAGGCCTAAGTGGACTTGAA2770.39295796626519697No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCACTGTTGAAGGTTG2430.3447248584925735No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGAAAGTTG2340.3319572711409967No Hit
GCTGCGTTCTTCATCGATGCGATAAAGATTGGCATCATGTTTAATGGGTG1640.23265381396206608No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA1580.22414208906101485No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG1570.22272346824417302No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT1280.18158346455575888No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCTCCTGTCCGAGGT1280.18158346455575888No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGCCGAGAGTCG980.13902484005050292No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT900.12767587351576798No Hit
GCTGCGTTCTTCATCGATTCGAGAGCCGAGATATCCGTTGCCGAGAGTCG850.12058276943155864No Hit
GCTGCGTTCTTCATCGATGCAGGTGAAGAGGTCTATGGCGGACAGGTCGT840.11916414861471676No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGATGCCGGACGATCGGGCAAACT820.11632690698103304No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCTGTTGCCGAGAGTCG810.11490828616419117No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTAAAAGTTG720.10214069881261437No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)